LEPR 重组蛋白, Mouse (His)

LEPR Protein, Mouse, Recombinant (His)

产品编号 TMPJ-00976

LEPR Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with C-10His tag. The predicted molecular weight is 120-150 KDa and the accession number is P48356.

规格	价格	库存
10 μg	¥ 820	5日内发货
50 μg	¥ 2,480	5日内发货
500 μg	¥ 12,400	5日内发货
1 mg	¥ 18,700	5日内发货

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常见问题解答

问超声应使用什么样的频率？

答频率建议使用常规的超声清洗频率即可，一般在20 kHz - 100 kHz。

问加药后检测结果没有作用，是不是产品质量有问题？

答产品质量没有问题，都是经过两次严格质检的，HNMR确定产品结构，HPLC确定化合物纯度。同一个抑制剂由于实验材料（细胞、动物）的不同，IC50 和实验效果也是不一样的，不能照搬文献报道的方法，应该根据自己的实验设立浓度梯度，得到一个最适合的浓度。

问准备进行动物实验，收到大包装粉末后，如何分装溶解？

答根据配制出来的溶液是不是澄清的确定一次性能配多少：如果配制出来是澄清溶液，可以一次性多配制一些，储存于4度冰箱，大概一周配一次，放久了可能会失效；如果配制出来是混悬液，建议每次使用的时候都现配现用。

问产品的溶解度是多少？

答建议您先查询TargetMol官网、陶术官网，看该抑制剂的产品介绍，找到溶解度信息。如果没有相应信息，建议联系技术对该化合物的溶解度进行测定。

问Tween-20 可以用 Tween-80 替代吗？

答不建议使用 Tween-20。相较于 Tween-20，Tween-80 的耐受性更好，Tween-80已被用作评估实验药物和毒物的行为影响的工具，且没有明显的副作用。参考文献：Castro CA， Hogan JB， Benson KA， Shehata CW， Landauer MR. Behavioral effects of vehicles: DMSO， ethanol， Tween-20， Tween-80， and emulphor-620. Pharmacol Biochem Behav. 1995 Apr;50(4):521-6.

产品信息

生物活性	Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
产品描述	LEPR Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with C-10His tag. The predicted molecular weight is 120-150 KDa and the accession number is P48356.
种属	Mouse
表达系统	HEK293 Cells
标签	C-10xHis
蛋白编号	P48356
别名	OB-R,OBR,OB receptor,Leptin receptor,LEP-R,LEPR,HuB219,DB,CD295
氨基酸序列	Leu22-Gly839
蛋白构建	Leu22-Gly839
蛋白纯度	Greater than 90% as determined by reducing SDS-PAGE. (QC verified)
分子量	120-150 KDa (reducing condition)
内毒素	< 0.1 ng/µg (1 EU/µg) as determined by LAL test.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4.
复溶方法	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
研究背景	The Leptin receptor is a member of the Class I cytokine receptor family. It mediates the activities of Leptin, a multi-functional hormone produced primarily by adipose tissues that plays roles in food intake, energy metabolism, angiogenesis, reproduction, hematopoiesis, bone metabolism, and immune function. The human Leptin R gene encodes 1165 amino acids (aa) including a signal peptide, an extracellular region with cytokine receptor homology (CRH), multiple fibronectin type III domains and a WSXWS motif, a transmembrane domain, and a cytoplasmic domain that supports JAK/STAT signaling. Soluble Leptin R is the primary Leptin-binding protein in blood, where it maintains a pool of available bioactive Leptin, delays Leptin clearance from circulation, and down-regulates blood-brain transmission of Leptin. In humans, soluble Leptin R levels are inversely proportional to adiposity and are elevated in females versus males. Soluble Leptin R is also found up-regulated in patients with chronic heart failure, end-stage renal disease, and anorexia.It is expressed by tumor-initiating stem cells, and is proposed as a link between cancer and obesity.

LEPR Protein, Mouse, Recombinant (His)

LEPR Protein, Mouse, Recombinant (His)

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