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IGFBP-3 Protein, Human, Recombinant (His) V2 is expressed in HEK293 Cells. The accession number is AAH18962.

| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 5 μg | ¥ 496 | 6-8日内发货 | |
| 10 μg | ¥ 791 | 6-8日内发货 | |
| 20 μg | ¥ 1,330 | 5日内发货 | |
| 50 μg | ¥ 2,650 | 5日内发货 | |
| 100 μg | ¥ 4,470 | 5日内发货 | |
| 200 μg | ¥ 7,300 | 5日内发货 | |
| 500 μg | ¥ 13,900 | 5日内发货 |
| 生物活性 | Immobilized Human IGFBP-3, His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Human IGF-II, hFc Tag with the EC50 of 0.19 μg/ml determined by ELISA (QC Test). Human IGFBP-3, His Tag captured on CM5 Chip via anti-his antibody can bind Human IGFI, hFc Tag with an affinity constant of 2.32 nM as determined in SPR assay (Biacore T200). |
| 产品描述 | IGFBP-3 Protein, Human, Recombinant (His) V2 is expressed in HEK293 Cells. The accession number is AAH18962. |
| 种属 | Human |
| 表达系统 | HEK293 Cells |
| 标签 | C-His |
| 蛋白编号 | AAH18962 |
| 别名 | insulin-like growth factor binding protein 3,IGFBP3,IBP3,BP-53 |
| 蛋白构建 | Gly28-Lys291 |
| 蛋白纯度 | > 95% as determined by Tris-Bis PAGE |
| 分子量 | 29.8 kDa (Predicted); 45-60 kDa (Due to glycosylation) |
| 内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
| 蛋白性状 | Lyophilized powder |
| 缓冲液 | Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| 复溶方法 | Reconstitute the lyophilized protein in sterile deionized water. The product concentration should not be less than 100 μg/mL. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| 存储 | Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| 运输方式 | In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice. |
| 研究背景 | Insulin-like growth factor binding protein-3 (IGFBP-3) is a p53 tumor suppressor-regulated protein and a major carrier for IGFs in circulation. Among six high-affinity IGFBPs, which are IGFBP-1 through 6, IGFBP-3 is the most extensively investigated IGFBP species with respect to its IGF/IGF-I receptor (IGF-IR)-independent biological actions beyond its endocrine/paracrine/autocrine role in modulating IGF action in cancer. |