生物活性

产品描述	HLA-A02:01&B2M&MART-1 (ELAGIGILTV) Monomer Protein, Human, MHC (His & Avi), Biotinylated is expressed in HEK293 Cells. The accession number is A0A140T913(HLA-A02:01)&P61769(B2M)&ELAGIGILTV.
生物活性	Immobilized Biotinylated Human HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Monomer, His-Avi Tag at 0.5 μg/ml(100 μl/well) on the streptavidin precoated plate (5 μg/ml). Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 8.9 ng/ml determined by ELISA.
研究背景	Melanoma antigen recognized by T cell-1 (Mart-1), one of the melanosome-specific proteins, also recognized by cytotoxicity T lymphocytes as a marker. Mart-1 is considered to play a critical role in the immunotherapy for melanoma.
种属	Human
表达系统	HEK293 Cells
标签	C-His-Avi
蛋白编号	A0A140T913 (HLA-A*02:01) & P61769 (B2M) & ELAGIGILTV
蛋白构建	Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and ELAGIGILTV peptide
蛋白纯度	> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
缓冲液	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
复溶方法	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
内毒素	< 1 EU/μg of the protein as determined by the LAL method.

化学信息

分子量	50.40 kDa (Predicted); 53-63 kDa (Reducing condition, due to glycosylation)

储存&溶解度

运输方式

In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.

存储

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

实际储存温度请以COA为准

**HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Monomer Protein, Human, MHC (His & Avi), Biotinylated**

HLA-A*02:01&B2M&MART-1 (ELAGIGILTV) Monomer Protein, Human, MHC (His & Avi), Biotinylated

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