规格

价格

库存

数量

5 μg

¥ 559

5日内发货

10 μg

¥ 863

5日内发货

20 μg

¥ 1,350

5日内发货

50 μg

¥ 2,470

5日内发货

100 μg

¥ 3,920

5日内发货

200 μg

¥ 6,250

5日内发货

500 μg

¥ 11,600

5日内发货

生物活性

产品描述	HLA-A02:01&B2M&CMV pp65 (NLVPMVATV) Monomer Protein, Human, MHC (E. coli, His & Avi), Biotinylated is expressed in E. coli. The accession number is A0A140T913(HLA-A02:01)&P61769(B2M)&NLVPMVATV.
生物活性	Immobilized Biotinylated HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Monomer, His Tag at 1 μg/ml(100 μl/well) on the streptavidin precoated plate (5 μg/ml). Dose response curve for Anti-B2M Antibody, mFc Tag with the EC50 of 12.1 ng/ml determined by ELISA.
研究背景	Human cytomegalovirus (CMV), a β-herpes virus with a double-stranded DNA, infects a wide variety of cells and establishes latency in the host. CMVpp65, a tegument protein of the herpes virus CMV, is the main viral antigen found in peripheral blood mononuclear cells (PBMCs) after viral infection and may activate cell-mediated immunity, accounting for 70-90% of the cytotoxic CD8+ T cells' (CTLs) response to CMV. Among the pp65-derived CTL epitope peptides, the 9-mer peptide 495NLVPMVATV503 (CMVpp65 495-503 peptide) is the most immunogenic T cell epitope predominantly displayed on HLA-A*02:01, the most common MHC-I allele in the population.
种属	Human
表达系统	E. coli
标签	C-His-Avi
蛋白编号	A0A140T913(HLA-A*02:01)&P61769(B2M)&NLVPMVATV
蛋白构建	Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and NLVPMVATV peptide
蛋白纯度	> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
缓冲液	Lyophilized from 0.22 μm filtered solution in 20 mM Tris, 200 mM NaCl (pH 8.0). Normally 8% mannitol is added as protectant before lyophilization.
复溶方法	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
别名	NLVPMVATV, CMVpp65
内毒素	< 1 EU/μg of the protein as determined by the LAL method.

化学信息

分子量	35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M)

储存&溶解度

运输方式

In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.

存储

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

实际储存温度请以COA为准

**HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Monomer Protein, Human, MHC (E. coli, His & Avi), Biotinylated**

HLA-A*02:01&B2M&CMV pp65 (NLVPMVATV) Monomer Protein, Human, MHC (E. coli, His & Avi), Biotinylated

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