GPHA2 Protein, Human, Recombinant (mFc) is expressed in HEK293 mammalian cells with mFc tag. The predicted molecular weight is 38.1 kDa and the accession number is Q96T91.
生物活性 | Testing in progress |
产品描述 | GPHA2 Protein, Human, Recombinant (mFc) is expressed in HEK293 mammalian cells with mFc tag. The predicted molecular weight is 38.1 kDa and the accession number is Q96T91. |
种属 | Human |
表达系统 | HEK293 Cells |
标签 | C-mFc |
蛋白编号 | Q96T91 |
别名 | ZSIG51, glycoprotein hormone α 2, A2, GPA2, glycoprotein hormone alpha 2 |
蛋白构建 | The Human GPHA2 (Q96T91) (Met1-Tyr129) was fused with Fc region of Mouse IgG1 at the C-terminus. |
蛋白纯度 | > 85 % as determined by SDS-PAGE |
分子量 | 38.1 kDa (predicted) |
内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted protein solutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | GPHA2 is a member of the glycoprotein hormones subunit alpha family. Glycoprotein hormones consist of two subunits, the common alpha- and specific beta-subunits, which associate noncovalently to form a heterodimer. The alpha-subunit combines with four distinct beta-subunits giving rise to four biologically active hormones in the human: FSH, LH, TSH, and CG. GPHA2 and glycoprotein hormone beta 5 (GPHB5) can form a noncovalent heterodimer. GPHA2 can be detected in a variety of tissues. Recombinant A2/B5 heterodimeric glycoproteins activate human TSH receptors, but not LH and FSH receptors, and shows high affinity to TSH receptors in a radioligand receptor assay. The heterodimer also stimulates cAMP production and thymidine incorporation by cultured thyroid cells and increases serum thyroxine levels in TSH-suppressed rats in vivo. This new heterodimeric glycoprotein hormone was named Thyrostimulin based on its thyroid-stimulating activity. The expression of Thyrostimulin in the anterior pituitary known to express TSH receptors suggested a paracrine mechanism. |
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GPHA2 Protein, Human, Recombinant (mFc) ZSIG51 A 2 GPA 2 glycoprotein hormone α 2 A2 GPA2 ZSIG 51 glycoprotein hormone alpha 2 A-2 ZSIG-51 glycoprotein hormone a 2 GPA-2 recombinant recombinant-proteins proteins protein