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GCG Protein, Human, Recombinant (His)

产品编号 TMPJ-00742

Glucagon is a secreted protein and belongs to the glucagon family. Glucagon can be cleved into 8 chains, playing an important role in initiating and maintaining hyperglycemic conditions in diabetes. Glucagon can regulates blood glucose by decreasing glycolysis and increasing gluconeogenesis. In addition, Glucagon is involved in initiating and maintaining hyperglycemic conditions in diabetes. Glucagon release is stimulated by hypoglycemia and inhibited by hyperglycemia, insulin, and somatostatin. In the glucagon antagonist, His-53 and Phe-58 are missing. This antagonist has been successfully utilized to reduce glucose concentration in vivo.

GCG Protein, Human, Recombinant (His)

GCG Protein, Human, Recombinant (His)

产品编号 TMPJ-00742
Glucagon is a secreted protein and belongs to the glucagon family. Glucagon can be cleved into 8 chains, playing an important role in initiating and maintaining hyperglycemic conditions in diabetes. Glucagon can regulates blood glucose by decreasing glycolysis and increasing gluconeogenesis. In addition, Glucagon is involved in initiating and maintaining hyperglycemic conditions in diabetes. Glucagon release is stimulated by hypoglycemia and inhibited by hyperglycemia, insulin, and somatostatin. In the glucagon antagonist, His-53 and Phe-58 are missing. This antagonist has been successfully utilized to reduce glucose concentration in vivo.
规格价格库存数量
10 μg
¥ 1,170
5日内发货
50 μg
¥ 3,470
5日内发货
500 μg
¥ 12,100
5日内发货
1 mg
¥ 17,400
5日内发货
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实验操作小课堂
常见问题解答
加药后检测结果没有作用,是不是产品质量有问题?
产品质量没有问题,都是经过两次严格质检的,HNMR确定产品结构,HPLC确定化合物纯度。同一个抑制剂由于实验材料(细胞、动物)的不同,IC50 和实验效果也是不一样的,不能照搬文献报道的方法,应该根据自己的实验设立浓度梯度,得到一个最适合的浓度。
溶解度写的“mg/mL”和“mM”是什么意思?
mg/mL 与 mM,是两种不同单位的表示方式,所指代的最大溶解度是一样的,可以通过官网网页下方的摩尔浓度计算器进行换算
如何选择合适的溶剂?
常用的溶剂包括DMSO、水、乙醇等。建议您根据抑制剂给出的溶解度参考,选择合适的溶剂。如果该抑制剂可以溶于DMSO,建议使用没有吸潮新开封的 DMSO,如果有潮气污染,很容易带来抑制剂降解或难溶的问题。如果该抑制剂可以溶于水,建议您根据实验类型选择无菌水、生理盐水、无菌 PBS 、培养基等作为溶剂。
得到的杀死细胞的 IC50 与网页上 IC50 差距较大怎么办?
您所测试的IC50是指细胞的增殖抑制实验(半抑制率),细胞增殖抑制实验 IC50值,和网页上的靶点IC50其实是不同的意义。文献里和网页上 IC50其实往往是指对于靶点无细胞实验的抑制率,这样的实验一般是通过激酶或者蛋白纯化实验做出来的,nM 级别的浓度比较常见,但是细胞的增殖抑制实验 IC50 是指细胞的半致死率,同时牵涉到细胞的代谢以及渗透,一般浓度会高一些。 另外,同一个化合物对不同细胞模型的效果也是不同的,建议尝试增加孵育量、延长孵育时间。
如何设置母液浓度?
母液浓度需低于官网给出的溶解度,在这个范围内,根据工作液浓度设置母液浓度,细胞实验中,建议母液浓度设定在工作液浓度的 1000 倍以上。
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产品信息

生物活性
Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
产品描述
Glucagon is a secreted protein and belongs to the glucagon family. Glucagon can be cleved into 8 chains, playing an important role in initiating and maintaining hyperglycemic conditions in diabetes. Glucagon can regulates blood glucose by decreasing glycolysis and increasing gluconeogenesis. In addition, Glucagon is involved in initiating and maintaining hyperglycemic conditions in diabetes. Glucagon release is stimulated by hypoglycemia and inhibited by hyperglycemia, insulin, and somatostatin. In the glucagon antagonist, His-53 and Phe-58 are missing. This antagonist has been successfully utilized to reduce glucose concentration in vivo.
种属
Human
表达系统
HEK293 Cells
标签C-6xHis
蛋白编号P01275
别名
Oxyntomodulin,OXY,OXM,Incretin Hormone,GRPP,Glucagon-Like Peptide 2,Glucagon-Like Peptide 1,Glucagon,GLP-2,GLP-1,Glicentin-Related Polypeptide,Glicentin,GCG
氨基酸序列
Arg21-Lys180
蛋白构建
Arg21-Lys180
分子量19 KDa (reducing condition)
内毒素< 0.1 ng/µg (1 EU/µg) as determined by LAL test.
缓冲液Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 200 mM NaCl, 1 mM DTT, 50% Glycerol, pH 8.0.
存储
Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
研究背景
Glucagon is a secreted protein and belongs to the glucagon family. Glucagon can be cleved into 8 chains, playing an important role in initiating and maintaining hyperglycemic conditions in diabetes. Glucagon can regulates blood glucose by decreasing glycolysis and increasing gluconeogenesis. In addition, Glucagon is involved in initiating and maintaining hyperglycemic conditions in diabetes. Glucagon release is stimulated by hypoglycemia and inhibited by hyperglycemia, insulin, and somatostatin. In the glucagon antagonist, His-53 and Phe-58 are missing. This antagonist has been successfully utilized to reduce glucose concentration in vivo.

SCI 文献

计算器

  • 复溶 计算器
  • 重组蛋白稀释 计算器
  • 比活力 计算器

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