Fc gamma RIIA/CD32a H167 重组蛋白, Human (TMPK-00211)

Fc gamma RIIA/CD32a (H167) Protein, Human, Recombinant (His & Avi), Biotinylated

产品编号 TMPK-00211

Fc gamma RIIA/CD32a (H167) Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 23.2 kDa and the accession number is P12318-1.

规格	价格	库存
100 μg	¥ 5,180	5日内发货
500 μg	¥ 20,700	5日内发货
1 mg	¥ 34,600	5日内发货

大包装 & 定制

加入购物车

TargetMol 的所有产品仅用作科学研究或药证申报，不能被用于人体，我们不向个人提供产品和服务。请您遵守承诺用途，不得违反法律法规规定用于任何其他用途。

常见问题解答

问溶解度中写的“< 1 mg/mL refers to the slightly soluble or insoluble ”是什么意思？

答这句话指的是，如果溶解度 < 1 mg/mL，那我们就认为它是微溶或不溶的，这是针对写的“DMSO：Insoluble”或“DMSO：slightly soluble”这类化合物的。

问动物不能耐受 DMSO，给药时 DMSO量应该如何控制？

答对于普通的老鼠，DMSO 的浓度应控制在 10% 以下，对于裸鼠、转基因小鼠、耐受性弱的老鼠等，DMSO 浓度需控制在2%以下。对于首次操作的抑制剂，建议先做溶剂阴性对照，确认溶解对动物无非特异性影响。

问抑制剂能否用于细胞实验？

答可以的，我们的抑制剂都可以用于细胞/体外实验。但有些化合物还没有用于细胞实验的文献，这种情况下，建议您先做预实验，确保研究的可行性。

问超声应使用什么仪器？

答建议使用“超声清洗仪”，不推荐用“超声破碎仪”。

问细胞实验中需要加多少抑制剂？

答通常建议您参考同模型实验发表的文献报道；除参考文献报道以外，还需要通过预实验做“剂量-效应曲线”确定最佳作用浓度（浓度梯度）；通过“时间-效应曲线”确定最佳孵育时间（处理时间梯度）。另外，抑制剂的使用量受多种因素影响，包括抑制对象的可接触性、细胞通透性、孵育时间、细胞种类等等。我们建议通过检索文献确定使用抑制剂的起始浓度。如果有报道的 Ki 值或 IC50 值，可以采用其 5-10 倍的量开始尝试以达到抑制酶活性的最佳效果。如果抑制剂的 Ki 值或 IC50 值未知，则需要在更广泛的范围尝试抑制剂的使用浓度，并采用 Michaelis-Menten 动力学计算 Ki 值。一般设立溶解抑制剂时所采用的溶剂作为对照，以排除溶剂的非特异性影响。

产品信息

生物活性	Rituximab captured on CM5 Chip via Protein A can bind Biotinylated Human Fc gamma RIIA (H167) , His-Avi Tag with an affinity constant of 0.680 μM as determined in SPR assay.
产品描述	Fc gamma RIIA/CD32a (H167) Protein, Human, Recombinant (His & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-His-Avi tag. The predicted molecular weight is 23.2 kDa and the accession number is P12318-1.
种属	Human
表达系统	HEK293 Cells
标签	C-His-Avi
蛋白编号	P12318-1
别名	fcRII-a,FCRIIA,FcgRIIA,FCGR2A1,FCGR2A,FCGR2,FCG2,Fc γ RIIA,Fc gamma RIIA,CD32A
蛋白构建	Ala36-Ile218(H167)
蛋白纯度	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
分子量	23.2 kDa (predicted). Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.
内毒素	< 1 EU/μg by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
复溶方法	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10e-8-10e-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10e-6-10e-7 M) only recognizing IgG aggregates surrounding multivalent antigens.