ER beta 重组蛋白, Human (His)

ER beta Protein, Human, Recombinant (His)

产品编号 TMPH-01305

Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1/ER-alpha, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner.; Lacks ligand binding ability and has no or only very low ERE binding activity resulting in the loss of ligand-dependent transactivation ability. ER beta Protein, Human, Recombinant (His) is expressed in yeast with N-6xHis tag. The predicted molecular weight is 61.1 kDa and the accession number is Q92731.

ER beta Protein, Human, Recombinant (His)

产品编号 TMPH-01305

规格	价格	库存
20 μg	¥ 1,470	20日内发货
100 μg	¥ 2,970	20日内发货
1 mg	¥ 13,000	20日内发货

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常见问题解答

问Tween-20 可以用 Tween-80 替代吗？

答不建议使用 Tween-20。相较于 Tween-20，Tween-80 的耐受性更好，Tween-80已被用作评估实验药物和毒物的行为影响的工具，且没有明显的副作用。参考文献：Castro CA， Hogan JB， Benson KA， Shehata CW， Landauer MR. Behavioral effects of vehicles: DMSO， ethanol， Tween-20， Tween-80， and emulphor-620. Pharmacol Biochem Behav. 1995 Apr;50(4):521-6.

问抑制剂产品应如何储存？

答粉末储存在 -20℃，可以存 3 年以上。母液一般储存于 -80℃，可以存 1 年以上，建议多分装几管，避免反复冻融。常用的存于 4℃，可放一周以上。

问超声久了会不会对化合物的结构有影响？

答建议以较低频率对化合物进行超声，不会对化合物的结构造成影响。

问抑制剂是否可以高温灭菌？

答大部分产品均通过化学方法合成，反应条件一般在 50-80℃ 之间，因此不建议使用高温灭菌法进行灭菌，建议用 0.22 μm 滤膜过滤灭菌。

问细胞实验中，抑制剂如何溶解？

答用 DMSO 配置较高浓度母液，母液存于 -80℃，建议多分装几管，一周内使用的存于 4℃，避免反复冻融。实验时用 pbs、生理盐水、培养基、水这些稀释，细胞实验DMSO的终浓度不超过 0.1%。如果超过 0.1%，则需要在做预试验的时候，先进行溶剂阴性对照实验，排除溶剂对细胞的影响。

产品信息

生物活性	Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
产品描述	Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1/ER-alpha, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner.; Lacks ligand binding ability and has no or only very low ERE binding activity resulting in the loss of ligand-dependent transactivation ability. ER beta Protein, Human, Recombinant (His) is expressed in yeast with N-6xHis tag. The predicted molecular weight is 61.1 kDa and the accession number is Q92731.
种属	Human
表达系统	P. pastoris (Yeast)
标签	N-6xHis
蛋白编号	Q92731
别名	Nuclear receptor subfamily 3 group A member 2,NR3A2,Estrogen receptor beta,ESTRB,ESR2,ER-beta
氨基酸序列	DIKNSPSSLNSPSSYNCSQSILPLEHGSIYIPSSYVDSHHEYPAMTFYSPAVMNYSIPSNVTNLEGGPGRQTTSPNVLWPTPGHLSPLVVHRQLSHLYAEPQKSPWCEARSLEHTLPVNRETLKRKVSGNRCASPVTGPGSKRDAHFCAVCSDYASGYHYGVWSCEGCKAFFKRSIQGHNDYICPATNQCTIDKNRRKSCQACRLRKCYEVGMVKCGSRRERCGYRLVRRQRSADEQLHCAGKAKRSGGHAPRVRELLLDALSPEQLVLTLLEAEPPHVLISRPSAPFTEASMMMSLTKLADKELVHMISWAKKIPGFVELSLFDQVRLLESCWMEVLMMGLMWRSIDHPGKLIFAPDLVLDRDEGKCVEGILEIFDMLLATTSRFRELKLQHKEYLCVKAMILLNSSMYPLVTATQDADSSRKLAHLLNAVTDALVWVIAKSGISSQQQSMRLANLLMLLSHVRHASNKGMEHLLNMKCKNVVPVYDLLLEMLNAHVLRGCKSSITGSECSPAEDSKSKEGSQNPQSQ
蛋白构建	2-530 aa
蛋白纯度	> 90% as determined by SDS-PAGE.
分子量	61.1 kDa (predicted)
内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Tris-based buffer, 50% glycerol
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.
研究背景	Nuclear hormone receptor. Binds estrogens with an affinity similar to that of ESR1/ER-alpha, and activates expression of reporter genes containing estrogen response elements (ERE) in an estrogen-dependent manner.; Lacks ligand binding ability and has no or only very low ERE binding activity resulting in the loss of ligand-dependent transactivation ability.

ER beta Protein, Human, Recombinant (His)

ER beta Protein, Human, Recombinant (His)

产品信息

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