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EphB1 Protein, Mouse, Recombinant (His)

产品编号 TMPY-02206

别名: Cek6, Hek6, C130099E04Rik, AW488255, Elkh, Elk, ENSMUSG00000074119, Net, 9330129L11, EPH receptor B1

EphB1 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 59.6 kDa and the accession number is Q8CBF3-1.

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EphB1 Protein, Mouse, Recombinant (His)

规格	价格/CNY		货期	数量
200 μg	￥ 5,750		5日内发货

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参考文献

生物活性	Immobilized mouse EPHB1-His at 10 μg/ml (100 μl/well) can bind mouse EFNB1-Fc , The EC50 of mouse EFNB1-Fc is 0.04-0.08 μg/ml.
产品描述	EphB1 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 59.6 kDa and the accession number is Q8CBF3-1.

种属	Mouse
表达系统	HEK293 Cells
标签	C-His
蛋白编号	Q8CBF3-1
别名	Cek6, Hek6, C130099E04Rik, AW488255, Elkh, Elk, ENSMUSG00000074119, Net, 9330129L11, EPH receptor B1
蛋白构建	The Mouse EPHB1 (Q8CBF3-1) extracellular domain (Met 1-Leu 539) was expressed, with a polyhistidine tag at the C-terminus.
蛋白纯度	> 98 % as determined by SDS-PAGE
分子量	59.6 kDa (predicted)

内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	Ephrin type-B receptor 1, also known as EphB1, belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family which 16 known receptors (14 found in mammals) are involved: EPHA1, EPHA2, EPHA3, EPHA4, EPHA5, EPHA6, EPHA7, EPHA8, EPHA9, EPHA10, EPHB1, EPHB2, EPHB3, EPHB4, EPHB5, EPHB6. EphB2 receptor tyrosine kinase phosphorylates syndecan-2 and that this phosphorylation event is crucial for syndecan-2 clustering and spine formation. The Eph family of receptor tyrosine kinases (comprising EphA and EphB receptors) has been implicated in synapse formation and the regulation of synaptic function and plasticity6. Ephrin receptors are components of cell signalling pathways involved in animal growth and development, forming the largest sub-family of receptor tyrosine kinases (RTKs). Ligand-mediated activation of Ephs induces various important downstream effects and Eph receptors have been studied for their potential roles in the development of cancer. EphB receptor tyrosine kinases are enriched at synapses, suggesting that these receptors play a role in synapse formation or function. We find that EphrinB binding to EphB induces a direct interaction of EphB with NMDA-type glutamate receptors. This interaction occurs at the cell surface and is mediated by the extracellular regions of the two receptors, but does not require the kinase activity of EphB.

参考文献

1. Dalva MB, et al. (2000) EphB receptors interact with NMDA receptors and regulate excitatory synapse formation. Cell. 103(6): 945-56. 2. Takasu MA, et al. (2002) Modulation of NMDA receptor-dependent calcium influx and gene expression through EphB receptors. Science. 295(5554): 491-5. 3. Adams RH, et al. (1999) Roles of ephrinB ligands and EphB receptors in cardiovascular development: demarcation of arterial/venous domains, vascular morphogenesis, and sprouting angiogenesis. Genes Dev. 13(3): 295-306.

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Keywords

EphB1 Protein, Mouse, Recombinant (His) Cek6 AW 488255 Hek6 C130099E04Rik AW488255 Elkh ENSMUSG 00000074119 Elk AW-488255 ENSMUSG00000074119 Net 9330129L11 EPH receptor B1 ENSMUSG-00000074119 recombinant recombinant-proteins proteins protein

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