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DNA ligase Protein, Desulfotalea psychrophila, Recombinant (His) is expressed in E. coli.

| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 5 μg | ¥ 555 | 20日内发货 | |
| 10 μg | ¥ 890 | 20日内发货 | |
| 20 μg | ¥ 1,510 | 20日内发货 | |
| 50 μg | ¥ 2,270 | 20日内发货 | |
| 100 μg | ¥ 3,120 | 20日内发货 | |
| 200 μg | ¥ 4,790 | 20日内发货 | |
| 500 μg | ¥ 8,580 | 20日内发货 | |
| 1 mg | ¥ 13,300 | 20日内发货 |
| 生物活性 | Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first. |
| 产品描述 | DNA ligase Protein, Desulfotalea psychrophila, Recombinant (His) is expressed in E. coli. |
| 种属 | Desulfotalea psychrophila |
| 表达系统 | E. coli |
| 标签 | N-10xHis |
| 蛋白编号 | Q6API9 |
| 别名 | Polydeoxyribonucleotide synthase [NAD(+)],ligA,DNA ligase |
| 氨基酸序列 | RPQTSRLGRVNPVAEVEPTRLSGALIQRVTAHHYAMVRDRGIGPGARIRMSRSGEVIPKIEEVVKPVAAAQLDIPAHCPSCGSDLAWDGDYLFCLNNMSCPAQITNSMEHFFKILGNVDGFGPSSVQRLYEGGLVDLPALYAMESADFEQVGFGPKQATNMVEQLNRSREELIEDWRFLAAFGVHRLGMGNCEKLLRYVRLEDVFNLSEEQIVAEIKGFQEKTAHSICAGLAGIKDLFFQLFALGFKLEPTPLLAEGGQGEKSPISGKTIVFTGAMLAASRGDMEKQAKALGATVGKSITGKTDMLVTGQRVGATKMAKAETLGIAILSEEAYRQLVSLD |
| 蛋白构建 | 340-679 aa |
| 蛋白纯度 | > 85% as determined by SDS-PAGE. |
| 分子量 | 40.4 kDa (predicted) |
| 内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
| 缓冲液 | Tris-based buffer, 50% glycerol |
| 复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
| 存储 | Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| 运输方式 | In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice. |
| 研究背景 | DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA. |