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DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA. DNA ligase Protein, Colwellia psychrerythraea, Recombinant (His) is expressed in E. coli expression system with N-10xHis tag. The predicted molecular weight is 42.1 kDa and the accession number is Q47YI0.
别名 Polydeoxyribonucleotide synthase [NAD(+)], ligA, DNA ligase
DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA. DNA ligase Protein, Colwellia psychrerythraea, Recombinant (His) is expressed in E. coli expression system with N-10xHis tag. The predicted molecular weight is 42.1 kDa and the accession number is Q47YI0.


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| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 5 μg | ¥ 555 | 20日内发货 | |
| 10 μg | ¥ 890 | 20日内发货 | |
| 20 μg | ¥ 1,510 | 20日内发货 | |
| 50 μg | ¥ 2,270 | 20日内发货 | |
| 100 μg | ¥ 3,120 | 20日内发货 | |
| 200 μg | ¥ 4,790 | 20日内发货 | |
| 500 μg | ¥ 8,580 | 20日内发货 | |
| 1 mg | ¥ 13,300 | 20日内发货 |
| 产品描述 | DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA. DNA ligase Protein, Colwellia psychrerythraea, Recombinant (His) is expressed in E. coli expression system with N-10xHis tag. The predicted molecular weight is 42.1 kDa and the accession number is Q47YI0. |
| 生物活性 | Activity has not been tested. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first. |
| 研究背景 | DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA. |
| 种属 | Colwellia psychrerythraea |
| 表达系统 | E. coli |
| 标签 | N-10xHis |
| 蛋白编号 | Q47YI0 |
| 氨基酸序列 | KFPAEEELTCVEDVEFQVGRTGAITPVARLKPVFVGGVTVSNATLHNQDEITRLGLKVNDFVVIRRAGDVIPQIVSVVLDKRPDNAVDIVFPTSCPVCDSAVAKPEGEAVLRCTAGLFCAAQRKEAIKHFASRKAHDVDGLGDKLVEQLVDEKLINTPADLFKLTEIQVSTIDRMGKKSATNLINGLEQAKSTTLAKFIYGLGIREVGEATAANLANHFYTLAAIESASLEDLQNVSDVGEVVAKNIINFFKEEHNLAIVSGLSEVMHWPTIEIKSAEELPLAEQIFVLTGTLTQMGRTEAKTALQSLGAKVSGSVSKNTHFVVAGDKAGSKLTKAQDLGISVLTEDGLVALLAEHGITI |
| 蛋白构建 | 330-689 aa |
| 蛋白纯度 | > 85% as determined by SDS-PAGE. |
| 缓冲液 | Tris-based buffer, 50% glycerol |
| 复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
| 别名 | Polydeoxyribonucleotide synthase [NAD(+)], ligA, DNA ligase |
| 内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
| 分子量 | 42.1 kDa (predicted) |
| 运输方式 | In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice. |
| 存储 |
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