规格

价格

库存

数量

5 μg

¥ 258

6-8日内发货

10 μg

¥ 429

6-8日内发货

20 μg

¥ 683

5日内发货

50 μg

¥ 1,350

5日内发货

100 μg

¥ 2,630

5日内发货

200 μg

¥ 4,130

5日内发货

500 μg

¥ 8,580

5日内发货

1 mg

¥ 19,000

5日内发货

生物活性

产品描述	CD86 Protein, Mouse, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 26.19 kDa and the accession number is P42082-1.
生物活性	Immobilized Mouse B7-2, His Tag at 1μg/ml (100 μl/well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 7.2 ng/ml determined by ELISA. (QC Test) Loaded Human CTLA-4, hFc Tag on ProA-Biosensor can bind Mouse B7-2, His Tag with an affinity constant of 0.83 µM as determined in BLI assay .
研究背景	B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs). Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. B7-1 and B7-2 do not form hetero-oligomers, underscoring the biological relevance of dimeric and monomeric state of B7-1 and B7-2, respectively.
种属	Mouse
表达系统	HEK293 Cells
标签	C-His
蛋白编号	P42082-1
蛋白构建	Val24-Lys244
蛋白纯度	> 95% as determined by Bis-Tris PAGE, > 95% as determined by HPLC
蛋白性状	Lyophilized powder
缓冲液	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
复溶方法	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
别名	TS/A-2, MB7-2, MB7, Ly-58, Ly58, ETC-1, CLS1, CD86 antigen, Cd28l2, B7-2, B70, B7.2, B7
内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.

化学信息

分子量	26.19 kDa (predicted); 48-68 kDa (reducing condition, due to glycosylation)

储存&溶解度

运输方式

In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.

存储

It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

实际储存温度请以COA为准

B7-2/CD86 Protein, Mouse, Recombinant (His)

B7-2/CD86 Protein, Mouse, Recombinant (His)

产品介绍

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