CD47 Protein, Human, Recombinant (Avi & His), Biotinylated is expressed in HEK293 mammalian cells with C-Avi-6xHis tag. The predicted molecular weight is 30-40 KDa and the accession number is Q08722.
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 20 μg | ¥ 2,260 | 5日内发货 | |
| 100 μg | ¥ 6,130 | 5日内发货 |
| 生物活性 | Loaded Anti-Human CD47 mAb-Fc on Protein A Biosensor, can bind Human CD47-Avi-6His with an affinity constant of 0.20 nM as determined in BLI assay. (Regularly tested) |
| 产品描述 | CD47 Protein, Human, Recombinant (Avi & His), Biotinylated is expressed in HEK293 mammalian cells with C-Avi-6xHis tag. The predicted molecular weight is 30-40 KDa and the accession number is Q08722. |
| 种属 | Human |
| 表达系统 | HEK293 Cells |
| 标签 | C-Avi-6xHis |
| 蛋白编号 | Q08722 |
| 别名 | Protein MER6,MER6,Leukocyte Surface Antigen CD47,Integrin-Associated Protein,IAP,CD47,Antigenic Surface Determinant Protein OA3 |
| 氨基酸序列 | Gln19-Pro139 |
| 蛋白构建 | Gln19-Pro139 |
| 蛋白纯度 | Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
| 分子量 | 30-40 KDa (reducing condition) |
| 内毒素 | < 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
| 缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing 10 mMTris-Citrate, 150 mM NaCl, pH 8.0. |
| 复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
| 存储 | Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
| 运输方式 | In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice. |
| 研究背景 | CD47(Integrin-Associated Protein,IAP) is a 40 ‑ 60 kDa variably glycosylated atypical member of the immunoglobulin superfamily. The ubiquitously expressed CD47 binds to SIRP family members on macrophages, neutrophils, and T cells. CD47 is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The protein is also a receptor for the C-terminal cell-binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This protein has broad tissue distribution, and is reduced in expression on Rh erythrocytes. |
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