CD40 Ligand Protein, Human, Recombinant (His & Flag) is expressed in HEK293 mammalian cells with N-6xHis-Flag tag. The predicted molecular weight is 19-24 KDa and the accession number is P29965.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
10 μg | ¥ 550 | 5日内发货 | ||
50 μg | ¥ 1,670 | 5日内发货 | ||
500 μg | ¥ 13,700 | 5日内发货 | ||
1 mg | ¥ 20,500 | 5日内发货 |
产品描述 | CD40 Ligand Protein, Human, Recombinant (His & Flag) is expressed in HEK293 mammalian cells with N-6xHis-Flag tag. The predicted molecular weight is 19-24 KDa and the accession number is P29965. |
种属 | Human |
表达系统 | HEK293 Cells |
标签 | N-6xHis-Flag |
蛋白编号 | P29965 |
别名 | CD40LG, Tumor Necrosis Factor Ligand Superfamily Member 5, TRAP, CD40L, TNFSF5, T-Cell Antigen Gp39, CD154, TNF-Related Activation Protein, CD40-L, CD40 Ligand |
氨基酸序列 | Gly116-Leu261 |
蛋白构建 | Gly116-Leu261 |
蛋白纯度 | Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
分子量 | 19-24 KDa, reducing conditions |
内毒素 | < 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. |
复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
存储 |
Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice. |
研究背景 | CD40 Ligand (CD40LG) is a type II transmembrane glycoprotein that belongs to the TNF superfamily. Like other TNF superfamily members, CD40LG exists as a trimer in membrane bound and soluble form, both of which are bioactive. CD40LG is a ligand for CD40; its ligation also initiates signal transduction in CD40LG expressing cells. CD40LG is a differentiation antigen that is expressed on the surface of T-cells. It stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40LG has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T-cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL2. |
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CD40 Ligand Protein, Human, Recombinant (His & Flag) CD40LG Tumor Necrosis Factor Ligand Superfamily Member 5 TRAP CD 154 CD40L CD-154 TNFSF5 T-Cell Antigen Gp39 CD154 TNF-Related Activation Protein TNFSF 5 CD40-L CD40 Ligand TNFSF-5 recombinant recombinant-proteins proteins protein