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CD40 Ligand Protein, Human, Recombinant

CD40 Ligand Protein, Human, Recombinant

产品编号 TMPJ-00151
别名: CD40LG, CD40 Ligand, TRAP, TNF-Related Activation Protein, TNFSF5, Tumor Necrosis Factor Ligand Superfamily Member 5, T-Cell Antigen Gp39, CD40-L, CD154, CD40L

CD40 Ligand Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 15 KDa and the accession number is P29965.

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TargetMol
CD40 Ligand Protein, Human, Recombinant
规格 价格/CNY 货期 数量
10 μg ¥ 380 5日内发货
50 μg ¥ 1,090 5日内发货
500 μg ¥ 4,860 5日内发货
1 mg ¥ 6,950 5日内发货
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产品目录号及名称: CD40 Ligand Protein, Human, Recombinant (TMPJ-00151)
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生物活性
技术参数
产品性质
生物活性 1. Loaded Human CD40-His on HIS1K Biosensor, can bind Human CD40L with an affinity constant of 2.43 nM as determined in BLI assay. (Regularly tested) 2. Immobilized Human CD40-Fc at 5μg/ml (100 μl/well) can bind Human CD40L . The ED50 of Human CD40L is 6.05 ng/ml. (Regularly tested)
产品描述 CD40 Ligand Protein, Human, Recombinant is expressed in E. coli expression system. The predicted molecular weight is 15 KDa and the accession number is P29965.
种属 Human
表达系统 E. coli
标签 Tag Free
蛋白编号 P29965
别名 CD40LG, CD40 Ligand, TRAP, TNF-Related Activation Protein, TNFSF5, Tumor Necrosis Factor Ligand Superfamily Member 5, T-Cell Antigen Gp39, CD40-L, CD154, CD40L
氨基酸序列 Met113-Leu261
蛋白构建 Met113-Leu261
蛋白纯度 Greater than 95% as determined by reducing SDS-PAGE. Greater than 95% as determined by SEC-HPLC.
分子量 15 KDa (reducing condition)
内毒素 < 0.1 ng/µg (1 EU/µg) as determined by LAL test.
缓冲液 Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM PB, 200 mM NaCl, 0.1 mM EDTA, pH 7.0.
复溶方法 Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储

Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

运输方式

In general, Lyophilized powders are shipping with blue ice. Solutions are shipping with dry ice.

研究背景 CD40 Ligand (CD40LG) is a type II transmembrane glycoprotein that belongs to the TNF superfamily. Like other TNF superfamily members, CD40LG exists as a trimer in membrane bound and soluble form, both of which are bioactive. CD40LG is a ligand for CD40; its ligation also initiates signal transduction in CD40LG expressing cells. CD40LG is a differentiation antigen that is expressed on the surface of T-cells. It stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. CD40LG has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. It also co-stimulates proliferation of activated T-cells and this is accompanied by the production of IFN-gamma, TNF-alpha, and IL2.

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Keywords

CD40 Ligand Protein, Human, Recombinant CD-154 CD40LG CD40 Ligand TRAP TNF-Related Activation Protein TNFSF5 Tumor Necrosis Factor Ligand Superfamily Member 5 TNFSF-5 T-Cell Antigen Gp39 TNFSF 5 CD 154 CD40-L CD154 CD40L recombinant recombinant-proteins proteins protein

 

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