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顶部产品介绍化学信息储存和溶解信息计算器技术支持

CD3D & CD3E Heterodimer Protein, Human, Recombinant (Flag & His), Biotinylated

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SPR兼容缓冲液
货号 TMPY-05374

别名 T3D, IMD19, CD3-δ, CD3-DELTA, CD3d molecule, δ (CD3-TCR complex), CD3d molecule, delta (CD3-TCR complex)

CD3D & CD3E Heterodimer Protein, Human, Recombinant (Flag & His), Biotinylated is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 31.05 kDa and the accession number is P04234&P07766.

CD3D & CD3E Heterodimer Protein, Human, Recombinant (Flag & His), Biotinylated
其他形式的 “CD3D & CD3E Heterodimer Protein, Human, Recombinant (Flag & His), Biotinylated”:
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CD3 epsilon/CD3e Protein, Rat, Recombinant (hFc)
CD3 epsilon/CD3e Protein, Human, Recombinant (HEK293, His)
CD3D Protein, Human, Recombinant (His)
CD3D & CD3E Heterodimer Protein, Mouse, Recombinant (Fc-Flag & Fc-His)
CD3D & CD3E Heterodimer Protein, Human, Recombinant (hFc & His & Flag)
CD3 epsilon/CD3e Protein, Rhesus, Recombinant (hFc)
CD3 epsilon/CD3e Protein, Rhesus, Recombinant (His)
CD3D Protein, Cynomolgus, Recombinant (His)
CD3D Protein, Cynomolgus, Recombinant (hFc)
CD3D Protein, Mouse, Recombinant (hFc)
CD3D Protein, Human, Recombinant (hFc & FLAG)
CD3D & CD3E Heterodimer Protein, Mouse, Recombinant (Flag & His)
CD3D & CD3E Heterodimer Protein, Human, Recombinant (Flag & His)
CD3E & CD3G Heterodimer Protein, Human, Recombinant (Fc-Flag & mFc)

CD3D & CD3E Heterodimer Protein, Human, Recombinant (Flag & His), Biotinylated

一键复制产品信息
Rating icon 很棒
SPR兼容缓冲液
货号 TMPY-05374 别名 T3D, IMD19, CD3-δ, CD3-DELTA, CD3d molecule, δ (CD3-TCR complex), CD3d molecule, delta (CD3-TCR complex)

CD3D & CD3E Heterodimer Protein, Human, Recombinant (Flag & His), Biotinylated is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 31.05 kDa and the accession number is P04234&P07766.

规格价格库存数量
5 μg
¥ 873
3日内发货
10 μg
¥ 1,450
3日内发货
20 μg
¥ 2,430
现货
50 μg
¥ 3,990
5日内发货
100 μg
¥ 5,830
5日内发货
200 μg
¥ 9,950
5日内发货
500 μg
¥ 20,100
5日内发货
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纯度: SDS-PAGE: 98.4%; SEC-HPLC: 98.4%
颜色: 白色
性状: Lyophilized powder
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COA重组蛋白用户指南

产品介绍

生物活性
产品描述
CD3D & CD3E Heterodimer Protein, Human, Recombinant (Flag & His), Biotinylated is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 31.05 kDa and the accession number is P04234&P07766.
生物活性
1.Immobilized Recombinant Human CD3D & CD3E Heterodimer Protein, Biotinylated (Cat#TMPY-05374) at 2 μg/mL (100 μl/well) on Streptavidin precoated (2 μg/mL, 100 μL/well) can bind Anti-CD3 antibody, Mouse IgG2a (Muromonab), the EC50 is 1-3 ng/mL.
2.Amine-Labeled Biotinylated Recombinant Human CD3D & CD3E Heterodimer Protein, Flag & His Tag (Cat#TMPY-05374) captured on CM5 chip via Anti-His antibody, can bind OKT3 derived Anti-CD3 antibody, Mouse IgG2a with an affinity constant of 0.241 μM as determined in an SPR assay (Biacore T200) (Routinely tested).
种属
Human
表达系统
HEK293 Cells
标签C-Flag, C-His
蛋白编号P04234&P07766
蛋白构建A DNA sequence encoding the extracellular domain (Met1-Ala105) of human CD3D (P04234) was fused with a flag tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the extracellular domain (Met1-Asp126) of human CD3E (P07766) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the human CD3D/CD3E heterodimer was purified. The purified protein was biotinylated in vitro. Predicted N terminal: Phe 22 & Asp 23
蛋白纯度
> 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.
CD3D & CD3E Heterodimer Protein, Human, Recombinant (Flag & His), BiotinylatedCD3D & CD3E Heterodimer Protein, Human, Recombinant (Flag & His), Biotinylated
蛋白性状Lyophilized powder
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法Reconstituted with sterile deionized water to 0.25 mg/mL. Reconstitution conditions may vary depending on the lot.
别名T3D, IMD19, CD3-δ, CD3-DELTA, CD3d molecule, δ (CD3-TCR complex), CD3d molecule, delta (CD3-TCR complex)
内毒素< 1.0 EU/μg of the protein as determined by the LAL method.
化学信息
分子量31.05 kDa (predicted)
储存&溶解度
运输方式In general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
存储

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剂量转换

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技术支持

请阅读 重组蛋白用户指南 了解更多具体信息.

关键词

IMD-19IMD 19CD3-TCR complexCD3d molecule, delta
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