CD20 Protein, Human, Recombinant (Flag)

CD20 Protein, Human, Recombinant (Flag) is expressed in HEK293 mammalian cells with C-Flag tag. The predicted molecular weight is 33-37 kDa and the accession number is P11836.

规格	价格/CNY		数量
10 μg	￥ 2,960	5日内发货
50 μg	￥ 8,900	5日内发货
500 μg	￥ 47,100	5日内发货
1 mg	￥ 70,600	5日内发货

规格

价格/CNY

货期

数量

10 μg

￥ 2,960

5日内发货

50 μg

￥ 8,900

5日内发货

500 μg

￥ 47,100

5日内发货

1 mg

￥ 70,600

5日内发货

生物活性	1. Loaded Anti-Human CD20 mAb-Fc on AHC Biosensor, can bind Human CD20-Flag with an affinity constant of 3.47 pM as determined in BLI assay. (Regularly tested) 2. Immobilized Human CD20-Flag at 2μg/ml (100 μl/well) can bind Anti-Human CD20 mAb . The ED50 of Anti-Human CD20 mAb is 11.09 ng/ml. (Regularly tested)
产品描述	CD20 Protein, Human, Recombinant (Flag) is expressed in HEK293 mammalian cells with C-Flag tag. The predicted molecular weight is 33-37 kDa and the accession number is P11836.

种属	Human
表达系统	Human Cells
标签	C-Flag
蛋白编号	P11836
别名	B-lymphocyte surface antigen B1, B-lymphocyte antigen CD20, Membrane-spanning 4-domains subfamily A member 1, MS4A1, Leukocyte surface antigen Leu-16, Bp35, CD20
氨基酸序列	Met1-Pro297
蛋白构建	Recombinant Human B-lymphocyte antigen CD20 is produced by our Mammalian expression system and the target gene encoding Met1-Pro297 is expressed with a Flag tag at the C-terminus.
蛋白纯度	Greater than 70% as determined by reducing SDS-PAGE. (QC verified)
分子量	33-37 kDa, reducing conditions

内毒素	Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.
缓冲液	Supplied as a 0.2 μm filtered solution of 50mM HEPES, 150mM NaCl, 0.06% DDM, 0.012% CHS, 10% Glycerol, pH 7.4.
存储	Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles.
运输方式	The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below.
研究背景	CD20 is a 33-37 kDa non-glycosylated protein expressed on the surface of normal and malignant B lymphocytes, and belongs to the MS4A (membrane-spanning 4-domain family A) protein family. CD20 protein consists of four hydrophobic transmembrane domains, one intracellular and two extracellular domains (large and small loops) with both N- and C- termini residing within the cytosol. CD20 is also known to be physically coupled to major histocompatibility complex class II (MHCII), CD40 molecule, BCR, and the C-terminal src kinase-binding protein (CBP) that interacts with Src kinases such as LYN, FYN, and LCK. CD20 deficiency resulted in a reduced number of circulating memory B cells, reduced isotype switching of Ig, and decreased IgG antibody levels. In agreement with this observation, challenging the patient’s primary B cells in vitro using T-dependent and Tindependent antigens led to the normal proliferation and secretion of IgM but reduced production of IgG.

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CD20 Protein, Human, Recombinant (Flag)

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