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B7-2/CD86 Protein, Human, Recombinant (hFc)

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B7-2/CD86 Protein, Human, Recombinant (hFc)

产品编号 TMPK-00882

B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs). Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. B7-1 and B7-2 do not form hetero-oligomers, underscoring the biological relevance of dimeric and monomeric state of B7-1 and B7-2, respectively. B7-2 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-hFc tag. The predicted molecular weight is 52 kDa and the accession number is P42081-1.

B7-2/CD86 Protein, Human, Recombinant (hFc)

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产品编号 TMPK-00882

规格	价格	库存
5 μg	¥ 239	6-8日内发货
10 μg	¥ 369	6-8日内发货
20 μg	¥ 595	5日内发货
50 μg	¥ 1,150	5日内发货
100 μg	¥ 1,920	5日内发货
200 μg	¥ 3,480	5日内发货
500 μg	¥ 7,710	5日内发货

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产品信息

生物活性	Immobilized Human CTLA-4, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human B7-2, hFc Tag with the EC50 of 125.7ng/ml determined by ELISA.
产品描述	B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs). Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. B7-1 and B7-2 do not form hetero-oligomers, underscoring the biological relevance of dimeric and monomeric state of B7-1 and B7-2, respectively. B7-2 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with C-hFc tag. The predicted molecular weight is 52 kDa and the accession number is P42081-1.
种属	Human
表达系统	HEK293 Cells
标签	C-hFc
蛋白编号	P42081-1
别名	MGC34413,LAB72,FUN-1,CD86 molecule,CD86,CD28LG2,BU63,B7-2 antigen,B7-2,B72,B70
蛋白构建	Leu26-Pro247
蛋白纯度	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
分子量	52 kDa (predicted). Due to glycosylation, the protein migrates to 75-90 kDa based on Tris-Bis PAGE result.
内毒素	< 1 EU/μg by the LAL method.
蛋白性状	Lyophilized powder
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
复溶方法	Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	B7-1 and B7-2 are homologous costimulatory ligands expressed on the surface of antigen presenting cells (APCs). Binding of these molecules to the T cell costimulatory receptors, CD28 and CTLA-4, is essential for the activation and regulation of T cell immunity. B7-1 and B7-2 do not form hetero-oligomers, underscoring the biological relevance of dimeric and monomeric state of B7-1 and B7-2, respectively.