ANXA2 Protein, Mouse, Recombinant (His) is expressed in E. coli expression system with N-His tag. The predicted molecular weight is 39.6 kDa and the accession number is P07356.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
100 μg | ¥ 2,660 | 现货 | ||
500 μg | ¥ 10,600 | 5日内发货 | ||
1 mg | ¥ 17,800 | 5日内发货 |
产品描述 | ANXA2 Protein, Mouse, Recombinant (His) is expressed in E. coli expression system with N-His tag. The predicted molecular weight is 39.6 kDa and the accession number is P07356. |
种属 | Mouse |
表达系统 | E. coli |
标签 | N-His |
蛋白编号 | P07356 |
别名 | LIP2PAP-IV, Annexin II, LPC2DP36, LPC2D, CAL1H, Annexin A2, ANX2L4, ANX2, PAP-IV, p36, Lipocortin II, Chromobindin-8, ANXA2 |
蛋白构建 | Ser2-Asp339 |
蛋白纯度 | > 95% as determined by Tris-Bis PAGE |
分子量 | 39.6 kDa (predicted) same as Tris-Bis PAGE result. |
内毒素 | < 1 EU/μg by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing 20 mM MES, 150 mM NaCl, 200 mM L-arginine, 5 mM DTT (pH 5.5). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
复溶方法 | Reconstitute the lyophilized protein in 20mM MES, 150mM NaCl, 200mM L-arginine, 5mM DTT (pH 5.5). The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | ANXA2, highly expressed in invasive breast cancer cells, is closely related with poor prognosis, and acts as a molecular switch to EGFR activation. ANXA2 expression is inversely correlated with cell sensitivity to gefitinib. Knockdown of ANXA2 expression in MDA-MB-231 cells increased the gefitinib induced cell death. When ANXA2 was overexpressed in MCF7 cells, the gefitinib induced cell death was decreased. Furthermore, the phosphorylation of ANXA2 at Tyr23 is negatively correlated with the sensitivity of TNBC to gefitinib. |
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ANXA2 Protein, Mouse, Recombinant (His) LIP2PAP-IV Annexin II ANX 2 LPC2DP36 ANXA-2 LPC2D CAL1H Annexin A2 ANXA 2 ANX2L4 ANX2 ANX-2 PAP-IV p36 Lipocortin II Chromobindin-8 ANXA2 recombinant recombinant-proteins proteins protein