4-1BB Ligand/TNFSF9 Protein, Human, Recombinant (His & Flag) is expressed in HEK293 mammalian cells with N-6xHis-Flag tag. The predicted molecular weight is 30 KDa and the accession number is P41273.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
10 μg | ¥ 550 | 5日内发货 | ||
50 μg | ¥ 1,670 | 5日内发货 | ||
500 μg | ¥ 13,700 | 5日内发货 | ||
1 mg | ¥ 20,500 | 5日内发货 |
生物活性 | Loaded Human 4-1BB-Fc-His on Protein A Biosensor, can bind Human 4-1BBL-His-Flag with an affinity constant of 4.53 pM as determined in BLI assay. (Regularly tested) |
产品描述 | 4-1BB Ligand/TNFSF9 Protein, Human, Recombinant (His & Flag) is expressed in HEK293 mammalian cells with N-6xHis-Flag tag. The predicted molecular weight is 30 KDa and the accession number is P41273. |
种属 | Human |
表达系统 | Human Cells |
标签 | N-6His-Flag |
蛋白编号 | P41273 |
别名 | Tumor necrosis factor ligand superfamily member 9, 4-1BBL, TNFSF9, 4-1BB ligand |
氨基酸序列 | Arg71-Glu254 |
蛋白构建 | Recombinant Human 4-1BB Ligand is produced by our Mammalian expression system and the target gene encoding Arg71-Glu254 is expressed with a 6His, Flag tag at the N-terminus. |
蛋白纯度 | Greater than 95% as determined by reducing SDS-PAGE. (QC verified) |
分子量 | 30 KDa, reducing conditions |
内毒素 | Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test. |
缓冲液 | Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 500mM NaCl, 5% Trehalose,5% Mannitol, 0.01% Tween80, 1mM EDTA, pH8.0. |
复溶方法 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
存储 |
Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
运输方式 |
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature listed below. |
研究背景 | Tumor necrosis factor ligand superfamily member 9(4-1BBL) is single-pass type II membrane protein which is a member of the the tumor necrosis factor family. 4-1BBL is a 254 amino acids cytokine that is expressed in brain, placenta, lung, skeletal muscle and kidney. TNFSF9 has been shown to reactivate anergic T lymphocytes in addition to promoting T lymphocyte proliferation. This cytokine may have a role in activation-induced cell death (AICD) and cognate interactions between T-cells and B-cells/macrophages. It has also been shown to be required for the optimal CD8 responses in CD8 T cells, and is thought to be involved in T cell-tumor cell interaction. |
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4-1BB Ligand/TNFSF9 Protein, Human, Recombinant (His & Flag) TNFSF 9 TNFSF-9 Tumor necrosis factor ligand superfamily member 9 4-1BBL TNFSF9 4-1BB ligand recombinant recombinant-proteins proteins protein