4-1BB Ligand/TNFSF9 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 47.9 kDa and the accession number is A0A0U5J8I0.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
100 μg | ¥ 3,430 | 现货 | ||
200 μg | ¥ 6,070 | 5日内发货 | ||
500 μg | ¥ 12,850 | 5日内发货 |
生物活性 |
1. Immobilized human TNFRSF9 at 10μg/mL (100μL/well) can bind human TNFSF9 , the EC50 of human TNFSF9 is 7-40ng/mL.
2. Measured by its ability to induce IL-8 secretion by HT1080 transfected with human CD137. The EC50 for this effect is typically 0.3-1.2 μg/mL.
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产品描述 | 4-1BB Ligand/TNFSF9 Protein, Human, Recombinant (hFc) is expressed in HEK293 mammalian cells with hFc tag. The predicted molecular weight is 47.9 kDa and the accession number is A0A0U5J8I0. |
种属 | Human |
表达系统 | HEK293 Cells |
标签 | N-hFc |
蛋白编号 | A0A0U5J8I0 |
别名 | CD137L, 4-1BB-L, tumor necrosis factor (ligand) superfamily, member 9, 4-1BBL |
蛋白构建 | The Human TNFSF9 (NP_003802.1) (Arg71-Glu254) was expressed with the Fc region of Human IgG1 at the N-terminus. |
蛋白纯度 |
> 85 % as determined by SDS-PAGE.
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分子量 | 47.9 kDa (predicted) |
内毒素 | < 1.0 EU/μg of the protein as determined by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization. |
复溶方法 | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | 4-1BB ligand is the high affinity ligand of 4-1BB, also known as CD137L or TNFSF9. It is shown to be a type II surface glycoprotein belonging to the TNF superfamily. Expression of 4-1BBL is restricted to APCs, such as dendritic cells, macrophages, and activated B cells. Members of the TNF-TNF receptor superfamily have been shown to play critical roles in regulating cellular activation, differentiation and apoptosis. Several studies have reported that 4-1BBL/4-1BB interaction provided a co-stimulatory signal to T cells, and increased T cell proliferation and cytokines production. In addition, 4-1BBL is involved in cancers, infectious diseases and autoimmune diseases.Cancer ImmunotherapyCo-stimulatory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint Detection: AntibodiesImmune Checkpoint Detection: ELISA AntibodiesImmune Checkpoint Detection: FCM AntibodiesImmune Checkpoint Detection: ICC AntibodiesImmune Checkpoint TargetsImmunotherapyTargeted Therapy |
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4-1BB Ligand/TNFSF9 Protein, Human, Recombinant (hFc) CD137L 4-1BB-L tumor necrosis factor (ligand) superfamily, member 9 4-1BBL recombinant recombinant-proteins proteins protein