4-1BB, is also known as CD137, is a type 2 transmembrane glycoprotein receptor belonging to the TNF superfamily.CD137 can be expressed by activated T cells, but to a larger extent on CD8 than on CD4 T cells. In addition, CD137 expression is found on dendritic cells, B cells, follicular dendritic cells, natural killer cells, granulocytes and cells of blood vessel walls at sites of inflammation. 4-1BB/CD137/TNFRSF9 Protein, Human, Recombinant (aa 24-186, hFc & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-hFc-Avi tag. The predicted molecular weight is 45.7 kDa and the accession number is Q07011.
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
100 μg | ¥ 5,180 | 5日内发货 | ||
500 μg | ¥ 20,700 | 5日内发货 | ||
1 mg | ¥ 34,600 | 5日内发货 |
生物活性 | Immobilized Biotinylated Human 4-1BB , hFc Tag at 1μg/ml (100μl/well) on the streptavidin precoated plate (5μg/ml). Dose response curve for Human 4-1BB Ligand (Trimer) , His Tag with the EC50 of 14.5ng/ml determined by ELISA. |
产品描述 | 4-1BB, is also known as CD137, is a type 2 transmembrane glycoprotein receptor belonging to the TNF superfamily.CD137 can be expressed by activated T cells, but to a larger extent on CD8 than on CD4 T cells. In addition, CD137 expression is found on dendritic cells, B cells, follicular dendritic cells, natural killer cells, granulocytes and cells of blood vessel walls at sites of inflammation. 4-1BB/CD137/TNFRSF9 Protein, Human, Recombinant (aa 24-186, hFc & Avi), Biotinylated is expressed in HEK293 mammalian cells with C-hFc-Avi tag. The predicted molecular weight is 45.7 kDa and the accession number is Q07011. |
种属 | Human |
表达系统 | HEK293 Cells |
标签 | C-hFc-Avi |
蛋白编号 | Q07011 |
别名 | TNFRSF9, FLJ43501, 4-1BB, 4-1BB ligand receptor, ILA, CD137, 41BB, CDw137, T cell antigen ILA |
蛋白构建 | Leu24-Gln186 |
蛋白纯度 | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
分子量 | 45.7 kDa (predicted). Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result. |
内毒素 | < 1 EU/μg by the LAL method. |
缓冲液 | Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization. |
复溶方法 | Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing. |
存储 |
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at-80℃. For reconstituted proteinsolutions, the solution can be stored at -20°c to -80'c for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots. |
运输方式 |
In general, Lyophilized powders are shipping with blue ice. |
研究背景 | 4-1BB, is also known as CD137, is a type 2 transmembrane glycoprotein receptor belonging to the TNF superfamily.CD137 can be expressed by activated T cells, but to a larger extent on CD8 than on CD4 T cells. In addition, CD137 expression is found on dendritic cells, B cells, follicular dendritic cells, natural killer cells, granulocytes and cells of blood vessel walls at sites of inflammation. |
bottom
您可能有的问题的答案可以在重组蛋白操作手册中找到
4-1BB/CD137/TNFRSF9 Protein, Human, Recombinant (aa 24-186, hFc & Avi), Biotinylated TNFRSF 9 CD-137 TNFRSF9 FLJ43501 4-1BB CD 137 4-1BB ligand receptor ILA CD137 41BB CDw137 FLJ-43501 TNFRSF-9 FLJ 43501 T cell antigen ILA recombinant recombinant-proteins proteins protein