WQ-3810 showed effective activity against Acinetobacter baumannii, including MDR isolates with MIC90 of 1 mg/L, eight times higher than ciprofloxacin (64 mg/L) and levofloxacin (8 mg/L). WQ-3810 also exhibits inhibitory effects on E. coli and S. pneumoniae, including FQR isolates, with MIC90 of 4 mg/L and 0.06 mg/L, respectively. WQ-3810 is the most potent among the fluoroquinolones tested against meticillin-resistant Staphylococcus aureus (MRSA) and Neisseria gonorrhoeae, including FQR isolates.