Tubulin-IN-64 is a sulfonated styrylquinazoline derivative that targets tubulin with high selectivity, exhibiting anticancer activity. It inhibits the EGFR/Akt/mTOR and EGFR/Ras pathways, inducing cell cycle arrest, apoptosis, and autophagy. In zebrafish glioblastoma xenograft models, Tubulin-IN-64 shows significant antitumor effects and is suitable for glioblastoma and leukemia research.