Tachyplesin-3 is a broad-spectrum cationic antimicrobial peptide. It exhibits inhibitory activity against Gram-positive bacteria, Gram-negative bacteria, fungi, and enveloped viruses. Tachyplesin-3 disrupts membrane integrity by binding to bacterial membrane lipopolysaccharides through its positive charge, leading to leakage of cellular contents. It also interferes with bacterial adhesion and aggregation, preventing biofilm formation, and shows a synergistic effect when used in combination with Piperacillin-Tazobactam (TZP).