store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Swinholide A 是可从海绵中提取的聚酮化合物,可于肌动蛋白结合,使肌动蛋白二聚化,Kd 约为 50 nM。Swinholide A 是一种细胞毒素,可稳定肌动蛋白二聚体并切断肌动蛋白丝。Swinholide A 具有抗真菌 (antifungal)活性,可破坏细胞的肌动蛋白细胞骨架。
产品描述 | Swinholide A is a polyketone compound that can be extracted from sponges and binds to actin to dimerize actin with Kd of about 50 nM. Swinholide A is a cytotoxin that stabilizes actin dimers and severs actin filaments. Swinholide A has antifungal activity and can damage the actin cytoskeleton of cells. |
体外活性 |
First isolated from the Okinawan marine sponge Theonella swinhoei, Swinholide A dimerizes actin[1]. Isolated from the marine sponge Theonella swinhoei, Swinholide A is highly cytotoxic to a variety of cancer cell lines[2]. Disrupting the actin cytoskeleton of cells grown in culture, Swinholide A sequesters actin dimers in vitro in both polymerizing and non-polymerizing buffers, with a binding stoichiometry of one Swinholide A molecule per actin dimer. Additionally, it rapidly severs F-actin in vitro with high cooperativity[2]. |
别名 | MK-7684 |
分子量 | 1389.87 |
分子式 | C78H132O20 |
CAS No. | 95927-67-6 |
store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
Methanol: Soluble
DMSO: Soluble
Ethanol: Soluble
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
Swinholide A 95927-67-6 Microbiology/Virology Antifungal MK-7684 MK 7684 MK7684 Inhibitor inhibitor inhibit