RT2 is a cationic amphiphilic antimicrobial peptide rich in tryptophan that primarily targets lipopolysaccharides (LPS) on bacterial surfaces and intracellular DNA. By binding to surface LPS, RT2 interacts with cytoplasmic membrane phospholipids, enabling rapid penetration of the bacterial cell membrane and subsequent binding to cytoplasmic components such as DNA, exerting its antibacterial effects. RT2 holds potential for research into acute and chronic infections caused by planktonic bacteria.