规格

价格

库存

数量

25 mg

待询

3-6月

50 mg

待询

3-6月

100 mg

待询

3-6月

生物活性

产品描述

Real Thiol, a reversible reaction-based fluorescent probe, can quantitatively monitor the real-time glutathione dynamics in living cells.

体外活性

Real Thiol (RT) shows ratiometric fluorescence responses with a wide dynamic range when reacting with Glutathione (GSH). Real Thiol and its GSH adduct (RT-GSH) show fluorescence maxima at 487 and 562?nm with excitation wavelengths at 405 and 488?nm, respectively. The Kd for the reaction between Real Thiol and GSH is 3.7?mM. The wide dynamic range of Real Thiol (1 to 10?mM) allows for monitoring of the GSH level changes in both directions in living cells. 10% of Real Thiol reacts with thiolated proteins while 90% of Real Thiol reacts with GSH [1].

化学信息

分子量	411.37
分子式	C₂₀H₁₇N₃O₇
CAS No.	2280796-90-7
Smiles	OC(=O)CN(CC(O)=O)C(=O)C(=C\c1cc2ccc(cc2oc1=O)N1CCC1)\C#N
密度	1.599 g/cm3 (Predicted)

储存&溶解度

存储

溶解度信息

DMSO: 101 mg/mL (245.52 mM), Sonication is recommended.

溶液配制表

DMSO

	1mg	5mg	10mg	50mg
1 mM	2.4309 mL	12.1545 mL	24.3090 mL	121.5451 mL
5 mM	0.4862 mL	2.4309 mL	4.8618 mL	24.3090 mL
10 mM	0.2431 mL	1.2155 mL	2.4309 mL	12.1545 mL
20 mM	0.1215 mL	0.6077 mL	1.2155 mL	6.0773 mL
50 mM	0.0486 mL	0.2431 mL	0.4862 mL	2.4309 mL
100 mM	0.0243 mL	0.1215 mL	0.2431 mL	1.2155 mL

该溶液配制表仅适用于固体产品。对于液体产品，请根据标明的浓度或密度计算稀释方案。

Real Thiol

Real Thiol

产品介绍

计算器

体内实验配液计算器

剂量转换

引用文献

关键词