keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
NEP, a turn-on fluorescent probe employing the intramolecular charge transfer (ICT) mechanism, facilitates the detection of vicinal dithiol-containing proteins (VDPs). Notably, NEP exhibits exceptional selectivity towards VDPs in live cells and in vivo and manifests a robust green fluorescence signal at λ ex /λ em =430/535 nm. Furthermore, NEP bears promise for the potential diagnosis and study of parkinsonism.
产品描述 | NEP, a turn-on fluorescent probe employing the intramolecular charge transfer (ICT) mechanism, facilitates the detection of vicinal dithiol-containing proteins (VDPs). Notably, NEP exhibits exceptional selectivity towards VDPs in live cells and in vivo and manifests a robust green fluorescence signal at λ ex /λ em =430/535 nm. Furthermore, NEP bears promise for the potential diagnosis and study of parkinsonism. |
体外活性 | NEP (1-30 μM; 6 hours) has no or little cytotoxicity in HepG2 and PC12 cells[1]. NEP (10 μM; 4 hours) causes the fluorescence intensity to decrease gradually in PC12 cells pretreated with 6-OHDA (0, 50, 100, and 200 μM; for 30 min)[1]. NEP contains a dithiarsolane moiety (five-membered As-S ring) as the receptor of VDPs. In the presence of VDPs, NEP displays a strong green fluorescence signal produced by the cyclic dithiarsolane cleavage and subsequent intramolecular cyclization to liberate the fluorophore. NEP maintains a reliable fluorescence response within the range of pH 7-10[1]. |
体内活性 | NEP (10 μM; for 4 h) causes the obvious green signal in zebrafishes (4 day old)[1]. |
别名 | VDP-green (NEP) |
分子量 | 654.63 |
分子式 | C29H31AsN4O5S2 |
CAS No. | 2414276-32-5 |
keep away from direct sunlight | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
NEP 2414276-32-5 VDP-green VDP-green (NEP) Inhibitor inhibitor inhibit