MMP-9-IN-14 is an MMP-9 inhibitor (IC50= 34.46 μM) that induces G1 phase cell cycle arrest and caspase-dependent apoptosis in cancer cells. It promotes the accumulation of phosphorylated γH2AX and inhibits the migration and invasion of cancer cells by downregulating the expression of MMP-2, MMP-9, and hTERT. In animal models, MMP-9-IN-14 suppresses tumor growth and angiogenic spreading. It is applicable in research on lung adenocarcinoma, cervical cancer, colorectal cancer, and other cancers.