FLT3-IN-41 is a potent FLT3 inhibitor with IC50 values of 3.16 nM for human FLT3-ITD and 294.7 nM for FLT3-WT. It suppresses the STAT5, Akt, and Erk signaling pathways by binding to the ATP pocket of FLT3-ITD and FLT3-WT, forming hydrogen bonds with hinge region residues and Phe830. FLT3-IN-41 induces G2/M phase arrest and promotes apoptosis in FLT3-ITD positive acute myeloid leukemia cells, demonstrating significant antiproliferative activity. This compound serves as an important tool for the study of acute myeloid leukemia.