EG01449 Free acid is a quinoline-based antagonist of neuropilin-1 (NRP1), exhibiting a Kd of 0.6 µM. It competitively inhibits the binding of VEGFA to NRP1, with an IC50 of 362 nM. By inhibiting NRP1-dependent signaling and reducing sodium currents in sensory neurons, EG01449 Free acid prevents VEGFA-induced pain. Additionally, it is capable of crossing the blood-brain barrier, allowing for targeted effects within the central nervous system. EG01449 Free acid is applicable for pain research.