Powder: -20°C for 3 years | In solvent: -80°C for 1 year
CXCR4 antagonist 7 (Compound PARA-B) 是可用于研究HIV 感染、炎症性疾病、癌症和 WHIM 综合症的CXCR4拮抗剂 (IC50 = 9.3 nM)。
规格 | 价格/CNY | 货期 | 数量 | |
---|---|---|---|---|
25 mg | ¥ 14,900 | 8-10周 | ||
50 mg | ¥ 19,420 | 8-10周 | ||
100 mg | ¥ 24,625 | 8-10周 |
产品描述 | CXCR4 antagonist 7 (Compound PARA-B) is able to be used in the HIV infection, inflammatory diseases, cancer, and WHIM syndrome research which is a antagonist of CXCR4 (IC 50 = 9.3 nM) [1]. |
体外活性 | CXCR4 antagonist 7 (PARA-B, 10 nM-1 μM, 20 h) inhibits CXCL12-induced GH4C1 cell proliferation with an IC 50 value of 9.3 nM [1]. CXCR4 antagonist 7 (1 μM, 12 h) inhibits CXCL12-dependent GH4C1 cell migration with inhibition rate of 50% [1]. CXCR4 antagonist 7 (50 nM, 30 min) reduces ERK1/2 phosphorylation induced by CXCL12 [1]. CXCR4 antagonist 7 (50 nM-1 μM, 30 min) acts via CXCR4 antagonism to revert CXCL12 induction of GH4C1 proliferation and migration [1]. Cell Viability Assay [1] Cell Line: GH4C1 cell (48 h of serum deprivation) Concentration: 1 μM Incubation Time: 24 h Result: Had no effect on cell viability of GH4C1 cell. Cell Proliferation Assay [1] Cell Line: GH4C1 cell ( FBS-starved GH4C1 cells treated with CXCL12 (25 nM) for 12 h) Concentration: 10 nM-1 μM Incubation Time: 20 h, 24 h Result: Inhibited proliferation of multiple cancer cell lines with IC 50 value ranging from 1.08 to 3.45 μM, and had no effect on cell viability of GH4C1cell. Cell Migration Assay [1] Cell Line: GH4C1 and GH4A11 cells (FBS-starved cells treated with CXCL12 (25 nM) for 48 h) Concentration: 50 nM-1 μM Incubation Time: 12 h for GH4C1, 30 min for GH4A11 Result: Reduced the number of migrating GH4C1 cells significantly, had no effect on GH4A11 cell (CRISPR-CAS9, reduction in CXCR4 mRNA) migration. Western Blot Analysis [1] Cell Line: GH4C1 cell (FBS-starved cells treated with CXCL12 (25 nM) for 15 min) Concentration: 50 nM Incubation Time: 30 min Result: Reduced ERK1/2 phosphorylation induced by CXCL12. |
分子量 | 315.33 |
分子式 | C15H17N5O3 |
CAS No. | 1185451-72-2 |
Powder: -20°C for 3 years | In solvent: -80°C for 1 year
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
CXCR4 antagonist 7 1185451-72-2 Inhibitor inhibitor inhibit