Antifungal agent 38 (50 μg/mL) has anti-fungal activity against 10 pathogenic fungi with inhibition rates of 56.57% ( R. solani ), 100% ( S. sclerotiorum ), 55.09% ( B. cinerea ), 71.23% ( F. graminearum ), 37.32% ( M. oryzae ), 50.87% ( P. capsici ), 100% ( A. flavus ), 59.75% ( P. expansum ), 100% ( M. fructicola ), 65.78% ( R. stolonifer ), respectively [1]. Antifungal agent 38 (5 μg/mL) damages plasma membrane integrity of M. fructicola [1]. Antifungal agent 38 (100 μg/mL) displays excellent anti-bacterial activity against X. oryzae accc 11602, X. axonpodis [1]. Antifungal agent 38 inhibits fungus growth with EC 50 s of 17.09 μg/mL ( R. solani ), 6.34 μg/mL ( S. sclerotiorum ), 25.24 μg/mL ( F. graminearum ), >10 μg/mL ( A. flavus ), 10.42 μg/mL ( M. fructicola ), 45.71 μg/mL ( B. cinerea ), respectively [1]. Cell Viability Assay [1] Cell Line: M. fructicola Concentration: 10, 25, 50 μg/mL Incubation Time: 2, 4, 6 days Result: Inhibited M. fructicola growth.

Antifungal agent 38 (compound S8) (200 μg/mL) shows equivalent curative and higher protective effects as the positive drug Thiophanate-Methyl in vivo [1]. Antifungal agent 38 (50, 100, 200 μg/mL, 30 mL; sprinkling) applies on pears and inhibits M. fructicola growth in vivo [1].