Anti-TSHR (CT) Polyclonal Antibody is a Rabbit antibody targeting TSHR (CT). Anti-TSHR (CT) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human (predicted:Rat,Chicken,Pig,Cow,Horse,Rabbit,GuineaPig)

1. Tissue/cell: human thyroid carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-TSHr (CT) Polyclonal Antibody, Unconjugated (TMAB-01912) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Paraformaldehyde-fixed, paraffin embedded (human parotid gland); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (TSHR) Polyclonal Antibody, Unconjugated (TMAB-01912) at 1:500 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
3. Sample:
Lane 1: Human Jurkat cell lysates
Lane 2: Human TT cell lysates
Primary: Anti-TSHR (CT) (TMAB-01912) at 1/2000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 86 kDa
Observed band size: 130 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human TSHR

Human

Thyroid axis,Hormones