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Anti-TROP-2 Antibody (7D257)
还可以产品编号 TMAY-00814
Anti-TROP-2 Antibody (7D257) 是一种 Mouse 抗体，靶向 TROP-2。Anti-TROP-2 Antibody (7D257) 可用于 WB,ELISA,ELISA(Det),IHC-P,FCM,ICC/IF。
Anti-TROP-2 Antibody (7D257)
Anti-TROP-2 Antibody (7D257)
还可以产品编号 TMAY-00814
Anti-TROP-2 Antibody (7D257) 是一种 Mouse 抗体，靶向 TROP-2。Anti-TROP-2 Antibody (7D257) 可用于 WB,ELISA,ELISA(Det),IHC-P,FCM,ICC/IF。
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-TROP-2 Antibody (7D257) is a Mouse antibody targeting TROP-2. Anti-TROP-2 Antibody (7D257) can be used in WB,ELISA,ELISA(Det),IHC-P,FCM,ICC/IF. |
| Ig Type | Monoclonal Mouse IgG1 |
| 克隆号 | 7D257 |
| 交叉反应 | Human, Rhesus, Cynomolgus Not React with:Mouse, Rat |
| 验证活性 | 1. Immunochemical staining of human TROP2 in human rectal cancer with mouse monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). 2. Immunochemical staining of human TROP2 in human placenta with mouse monoclonal antibody (1:60, formalin-fixed paraffin embedded sections). Positive staining was localized to membrane of cytotrophoblast. 3. Immunochemical staining of human TROP2 in human mammary gland with mouse monoclonal antibody (1:200, formalin-fixed paraffin embedded sections). Positive staining was localized to membrane of alveolus epithelium. 4. Flow cytometric analysis of human TROP2 expression on MCF-7 cells. Cells were stained with purified anti-Human TROP2, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells. 5. Anti-TROP2 mouse monoclonal antibody at 1:500 dilution. -Lane A: MCF7 Whole Cell Lysate. -Lane B: NCI-N87 Whole Cell lysate. -Lysates/proteins at 30 μg per lane. -Secondary -Goat Anti-Mouse IgG (H+L)/HRP at 1/10000 dilution. -Developed using the ECL technique. -Performed under reducing conditions. -Predicted band size:36 kDa. -Observed band size:36-40 kDa. 6. Immunochemical staining of monkey TROP2 in monkey Palm skin with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections. 7. Immunochemical staining of human TROP2 in human skin with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections. 8. Immunochemical staining of human TROP2 in human hysterocarcinoma with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections. 9. Immunochemical staining of human TROP2 in human skin cancer with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections. 10. Immunofluorescence staining of TROP-2 in NCI-N87 cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with mouse anti-Human TROP-2 monoclonal antibody (dilution ratio 1:60) at 4°C overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cell membrane. 11. Flow cytometric analysis of human TROP2 expression on NCI-N87 cells. Cells were stained with purified anti-Human TROP2, then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells. 12. Immunochemical staining of human TROP2 in human thyroid cancer with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections. |
| 应用 | WB,ELISA,ELISA(Det),IHC-P,FCM,ICC/IF |
| 推荐剂量 | WB: 1:500-1:2000; ELISA: 1:1000-1:2000; IHC-P: 1:100-1:500; ICC-IF: 1:20-1:100; FCM: 1:25-1:100; ELISA(Det): 1:1000-1:10000 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Mouse |
| 构建方式 | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human TROP2 extracellular domain (rh TROP2; TMPY-01262; NP_002344.2; Met 1-Thr 274). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | TROP-2, also referred to as tumor-associated calcium signal transducer 2 (TACSTD2), GA733-1 or M1S1, is a cell surface glycoprotein highly expressed in a wide variety of epithelial cancers. In contrast, there is little or no expression of Trop-2 in adult somatic tissue. Because it is a cell surface protein that is selectively expressed in tumor cells, Trop-2 is a potential therapeutic target. The cytoplasmic tail of Trop-2 possesses potential serine and tyrosine phosphorylation sites and a phosphatidyl-inositol binding consensus sequence. Trop-2 transduces an intracellular calcium signal, which are consistent with the hypothesis that it acts as a cell surface receptor and support a search for a physiological ligand. TROP2 encoding by an intronless gene was originally defined by the monoclonal antibody GA733, and is a member of a family of at least two type I membrane proteins. The other known member is GA733-2, also called EpCAM and TROP1. It has been suggested by studies that the GA733-1 gene was formed by the retroposition of the GA733-2 gene via an mRNA intermediate. |
| 偶联 | Unconjugated |
| 其他可选修饰符 | Biotin/FITC/HRP/PE |
| 可提供抗体种类 | 5 |
| 免疫原 | Recombinant Human TROP2 protein (TMPY-01262) |
| 抗原种属 | Human |
| 研究领域 | Cancer Drug Targets |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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