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Anti-TIE2 Antibody (9U826)
还可以产品编号 TMAY-01088
Anti-TIE2 Antibody (9U826) 是一种 Rabbit 抗体，靶向 TIE2。Anti-TIE2 Antibody (9U826) 可用于 ELISA(Cap),FCM,ICC/IF。
Anti-TIE2 Antibody (9U826)
Anti-TIE2 Antibody (9U826)
还可以产品编号 TMAY-01088
Anti-TIE2 Antibody (9U826) 是一种 Rabbit 抗体，靶向 TIE2。Anti-TIE2 Antibody (9U826) 可用于 ELISA(Cap),FCM,ICC/IF。
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产品介绍
生物活性
偶联与修饰
抗原信息
| 产品描述 | Anti-TIE2 Antibody (9U826) is a Rabbit antibody targeting TIE2. Anti-TIE2 Antibody (9U826) can be used in ELISA(Cap),FCM,ICC/IF. |
| Ig Type | Monoclonal Rabbit IgG |
| 克隆号 | 9U826 |
| 交叉反应 | Human |
| 验证活性 | 1. Immunofluorescence staining of Human Tie2 in HUVEC cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human Tie2 monoclonal antibody (1:60) at 37°C 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cell membrane and cytoplasm. 2. Flow cytometric analysis of Human TIE2(CD202b) expression on HUVEC cells. Cells were stained with purified anti-Human TIE2(CD202b), then a FITC-conjugated second step antibody. The histogram were derived from gated events with the forward and side light-scatter characteristics of intact cells. |
| 应用 | ELISA(Cap),FCM,ICC/IF |
| 推荐剂量 | ICC-IF: 1:20-1:100; FCM: 1:25-1:100; ELISA(Cap): 1:250-1:2000 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | This antibody was obtained from a rabbit immunized with purified, recombinant Human Tie2 / CD202b / TEK (rh Tie2 / CD202b / TEK; TMPY-01064; AAA61139.1; Met1-Lys745). |
| 纯化方式 | Protein A |
| 性状 | Liquid |
| 缓冲液 | 0.2 μm filtered solution in PBS |
| 研究背景 | TEK, or TIE-2, is an endothelial cell-specific receptor tyrosine kinase (RTK) that is known as a functioning molecule of vascular endothelial cells. TEK comprises a subfamily of RTK with TIE, and these two receptors play critical roles in vascular maturation, maintenance of integrity and remodeling. Targeted mutagenesis of both Tek and its agonistic ligand, Angiopoietin-1, result in embryonic lethality, demonstrating that the signal transduction pathways mediated by this receptor are crucial for normal embryonic development. TEK signaling is indispensable for the development of the embryonic vasculature and suggests that TEK signaling may also be required for the development of the tumor vasculature. |
| 偶联 | Unconjugated |
| 其他可选修饰符 | APC/FITC/PE |
| 可提供抗体种类 | 4 |
| 免疫原 | Recombinant Human Tie2 / CD202b / TEK protein (TMPY-01064) |
| 抗原种属 | Human |
| 研究领域 | Receptor Tyrosine Kinases (RTKs), Cancer Drug Targets, Hemangioblast Markers |
存储&运输
| 储存方式 | Store at 2°C-8°C for 1 month. Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. Preservative-Free. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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