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Anti-S100B Antibody (4A182)
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产品编号 TMAC-03670
别名 S100β, S100beta, S100-B, S100 calcium binding protein B, S100, NEF
Anti-S100B Antibody (4A182) 是一种 Rabbit 抗体，靶向 S100B。Anti-S100B Antibody (4A182) 可用于 WB,ICC/IF,IP,IHC。
Anti-S100B Antibody (4A182)
Anti-S100B Antibody (4A182)
还可以产品编号 TMAC-03670 别名 S100β, S100beta, S100-B, S100 calcium binding protein B, S100, NEF
Anti-S100B Antibody (4A182) 是一种 Rabbit 抗体，靶向 S100B。Anti-S100B Antibody (4A182) 可用于 WB,ICC/IF,IP,IHC。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,480 | 5日内发货 | |
| 100 μL | ¥ 2,480 | 5日内发货 |
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产品介绍
生物活性
偶联与修饰
抗原信息
化学信息
| 产品描述 | Anti-S100B Antibody (4A182) is a Rabbit antibody targeting S100B. Anti-S100B Antibody (4A182) can be used in WB,ICC/IF,IP,IHC. |
| 别名 | S100β, S100beta, S100-B, S100 calcium binding protein B, S100, NEF |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat,Goat,zebrafish |
| 验证活性 | 1. Western blot analysis of S100 beta on different lysates using anti-S100 beta antibody at 1/1,000 dilution. Positive control: Lane 1: Mouse liver, Lane 2: Mouse heart, Lane 3: Hela. 2. Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-S100 beta antibody. Counter stained with hematoxylin. 3. ICC staining S100 beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 4. ICC staining S100 beta in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 5. ICC staining S100 beta in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
| 应用 | |
| 推荐剂量 | WB: 1:1000-5000; IHC: 1:50-200; ICC/IF: 1:50-200 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | The family of EF-hand type Ca2+-binding proteins includes calbindin (previously designated vitamin D-dependent Ca2+-binding protein), S-100 α and β, calgranulins A (also designated MRP8), B (also designated MRP14) and C (S-100 like proteins), and the parvalbumin family members, including parvalbumin α and parvalbuminβ(also designated oncomodulin). The S-100 protein is involved in the regulation of cellular processes such as cell cycle progression and differentiation. Research also indicates that the S-100 protein may function in the activation of Ca2+ induced Ca2+ release, inhibition of microtubule assembly and inhibition of protein kinase C mediated phosphorylation. Two S-100 subunits, sharing 60% sequence identity, have been described as S-100 α chain and S-100 β chain. Three S-100 dimeric forms have been characterized, differing in their subunit composition of either two α chains, two β chains or one α and one β chain. S-100 localizes to the cytoplasm and nuclei of astrocytes, Schwann's cells, ependymomas and astrogliomas. S-100 is also detected in almost all benign naevi, malignant melanocytic tumours and in Langerhans cells in the skin. Calbindin, S-100 proteins and parvalbumin proteins are each expressed in neural tissues. In addition, S-100 α and β are present in a variety of other tissues, and calbindin is present in intestine and kidney. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 11 kDa. |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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