Anti-PKM2 Polyclonal Antibody is a Mouse antibody targeting PKM2. Anti-PKM2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,ICC/IF,IF.

IgG

Human,Mouse,Rat (predicted:Pig,Cow,Horse,Rabbit)

1. Sample:
MOLT-4 (Human) Cell Lysate at 30 μg
Primary: Anti-PKM2 (TMAB-01544) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 58 kDa
Observed band size: 58 kDa
2. Sample:
Hela Cell (Human) Lysate at 40 μg
NIH/3T3 Cell (Mouse) Lysate at 40 μg
Muscle (Mouse) Lysate at 40 μg
Jurkat Cell (Human) Lysate at 40 μg
Primary: Anti-PKM2 (TMAB-01544) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 58 kDa
Observed band size: 58 kDa
3. Tissue/cell: rat colitis tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-M2-PK Polyclonal Antibody, Unconjugated (TMAB-01544) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Mouse IgG, Cy5 conjugated at 37°C for 20 min;
Incubation: Anti-M2-PK Polyclonal Antibody, Unconjugated (TMAB-01544) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
5. Sample:
Lane 1: A549 (Human) Cell Lysate at 30 μg
Lane 2: U87MG (Human) Cell Lysate at 30 μg
Lane 3: U251 (Human) Cell Lysate at 30 μg
Primary: Anti-PKM2 (TMAB-01544) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa
6. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (PKM2) polyclonal Antibody, Unconjugated (TMAB-01544) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
7. U-87MG cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (PKM2) polyclonal Antibody, Unconjugated (TMAB-01544) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human PKM2

Human

Energy Metabolism,Energy Metabolism,Carbohydrate metabolism,Kinases/Phosphatases,Enzymes,Metabolism of carbohydrates