Anti-PKM2 Polyclonal Antibody is a Mouse antibody targeting PKM2. Anti-PKM2 Polyclonal Antibody can be used in ICC/IF,IF,IHC-Fr,WB.

IgG

Human,Mouse,Rat (predicted:Pig,Cow,Horse,Rabbit)

1. Sample:
MOLT-4 (Human) Cell Lysate at 30 μg
Primary: Anti-PKM2 (TMAB-01544) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 58 kDa
Observed band size: 58 kDa
2. Sample:
Hela Cell (Human) Lysate at 40 μg
NIH/3T3 Cell (Mouse) Lysate at 40 μg
Muscle (Mouse) Lysate at 40 μg
Jurkat Cell (Human) Lysate at 40 μg
Primary: Anti-PKM2 (TMAB-01544) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 58 kDa
Observed band size: 58 kDa
3. Sample:
Lane 1: A549 (Human) Cell Lysate at 30 μg
Lane 2: U87MG (Human) Cell Lysate at 30 μg
Lane 3: U251 (Human) Cell Lysate at 30 μg
Primary: Anti-PKM2 (TMAB-01544) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa
4. Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (PKM2) polyclonal Antibody, Unconjugated (TMAB-01544) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
5. U-87MG cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (PKM2) polyclonal Antibody, Unconjugated (TMAB-01544) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Mouse IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.

WB: 1:500-2000; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human PKM2

Human

PKM

Pyruvate kinase PKM

Energy Metabolism,Energy Metabolism,Carbohydrate metabolism,Kinases/Phosphatases,Enzymes,Metabolism of carbohydrates

Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.