Anti-Phospho-Cofilin (Ser3) Polyclonal Antibody is a Rabbit antibody targeting Phospho-Cofilin (Ser3). Anti-Phospho-Cofilin (Ser3) Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Mouse,Rat (predicted:Human,Pig,Cow,Horse,Sheep)

1. Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-Cofilin (Ser3)) Polyclonal Antibody, Unconjugated (TMAB-01400) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Phospho-Cofilin (Ser3)) Polyclonal Antibody, Unconjugated (TMAB-01400) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Sample:
Cerebrum (Mouse) Lysate at 40 μg
Primary: Anti-Phospho-Cofilin (Ser3) (TMAB-01400) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 18 kDa
Observed band size: 18 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated Synthesised phosphopeptide: human Cofilin around the phosphorylation site of Ser3

Human

Cell adhesion,Actins,Cytoskeleton,Axon marker,Axonal Guidance Proteins,Actin Binding Proteins