Anti-PDGF R beta/CD140b Polyclonal Antibody is a Rabbit antibody targeting PDGF R beta/CD140b. Anti-PDGF R beta/CD140b Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Rat (predicted:Mouse,Dog,Cow)

1. Sample:
DU145 (Human) Cell Lysate at 30 μg
Primary: Anti-PDGF Receptor beta (TMAB-01350) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 118 kDa
Observed band size: 118 kDa
2. Sample:
U251 (Human) Cell Lysate at 30 μg
Primary: Anti-PDGF Receptor beta (TMAB-01350) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 118 kDa
Observed band size: 118 kDa
3. Sample:
NIH/3T3 (Rat) Cell Lysate at 30 μg
Primary: Anti-PDGF Receptor beta (TMAB-01350) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 118 kDa
Observed band size: 118 kDa
4. Tissue/cell: Human brain glioma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-PDGFRB Polyclonal Antibody, Unconjugated (TMAB-01350) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human PDGF-R-B

Human

PDGF,Growth factor receptors,Growth Factors,Inflammatory mediators,SARS Coronavirus,PDGF,Receptor Tyrosine Kinases