Anti-NGF Polyclonal Antibody is a Rabbit antibody targeting NGF. Anti-NGF Polyclonal Antibody can be used in ICC/IF,IF,IHC-Fr,WB.

IgG

Human,Mouse,Rat (predicted:Dog,Pig,Cow,Sheep)

1. SH-SY5Y cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (NGFB) polyclonal Antibody, Unconjugated (TMAB-01236) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nucleus.
2. Sample:
Lane 1: Mouse Brain Lysates
Lane 2: Mouse Cerebellum Lysates
Lane 3: Human U-87 MG cell Lysates
Lane 4: Human SH-SY5Y cell Lysates
Lane 5: Human U-2 OS cell Lysates
Primary: Anti-NGFB (TMAB-01236) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 13/32 kDa
Observed band size: 13/32 kDa

WB: 1:500-2000; IHC-Fr: 1:100-500; ICC/IF: 1:100; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human NGF beta

Human

NGF

beta-nerve growth factor

Neurotrophins,Neuroregeneration

Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival.