Anti-MMP-13 Polyclonal Antibody 3 is a Rabbit antibody targeting MMP-13. Anti-MMP-13 Polyclonal Antibody 3 can be used in WB,IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat (predicted:Rabbit,Dog)

1. Sample:
Lane 1: Raw264.7 (Mouse) Cell Lysate at 30 μg
Lane 2: Jurkat (Human) Cell Lysate at 30 μg
Lane 3: MCF-7 (Human) Cell Lysate at 30 μg
Lane 4: Molt-4 (Human) Cell Lysate at 30 μg
Lane 5: A431 (Human) Cell Lysate at 30 μg
Primary: Anti-MMP13 (TMAB-01153R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kDa
Observed band size: 60 kDa
2. Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-MMP-13 Polyclonal Antibody, Unconjugated (TMAB-01153) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Tissue/cell: mouse lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-MMP-13 Polyclonal Antibody, Unconjugated (TMAB-01153) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
4. Paraformaldehyde-fixed, paraffin embedded (rat lung tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (MMP13) Polyclonal Antibody, Unconjugated (TMAB-01153) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 min and DAB staining.
5. Sample:
MG63 (Human) Cell Lysate at 30 μg
Primary: Anti-MMP13 (TMAB-01153) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kDa
Observed band size: 65 kDa

WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500

Polyclonal

KLH conjugated synthetic peptide: human MMP13

Human

MMPs,MMPs,MMPs,MMP,MMPs,Tumor biomarkers ELISA kits,MMP