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Anti-IL-2 Polyclonal Antibody

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Anti-IL-2 Polyclonal Antibody
产品编号 TMAB-00954

Anti-IL-2 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 IL-2。Anti-IL-2 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。

Anti-IL-2 Polyclonal Antibody

Anti-IL-2 Polyclonal Antibody

Rating icon 还可以
Anti-IL-2 Polyclonal Antibody
产品编号 TMAB-00954

Anti-IL-2 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 IL-2。Anti-IL-2 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。

规格价格库存数量
50 μL
¥ 1,165
5日内发货
100 μL
¥ 1,965
5日内发货
200 μL
¥ 2,780
5日内发货
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产品介绍

生物活性
产品描述
Anti-IL-2 Polyclonal Antibody is a Rabbit antibody targeting IL-2. Anti-IL-2 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
交叉反应
Human,Mouse,Rat
验证活性
1. Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (IL-2) Polyclonal Antibody, Unconjugated (TMAB-00954) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (IL-2) Polyclonal Antibody, Unconjugated (TMAB-00954) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
3. Sample:
Lane 1: Large intestine (Mouse) Lysate at 40 μg
Lane 2: Large intestine (Rat) Lysate at 40 μg
Primary: Anti-IL-2 (TMAB-00954) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 17 kDa
Observed band size: 23 kDa
应用
推荐剂量
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Secreted.
构建方式Hybridoma Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究背景The protein encoded by this gene is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli. [provided by RefSeq, Jul 2008]
抗原信息
免疫原
KLH conjugated synthetic peptide: human IL-2
抗原种属
Human
基因ID
Uniprot ID
研究领域
Interleukins,Regulatory T Cells,CD,Cytokines,Interleukins,Neurodegenerative disease
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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