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Anti-HIF1A Antibody (2X244)
还可以产品编号 TMAH-00543
别名 PASD8, MOP1, hypoxia inducible factor 1, α subunit (basic helix-loop-helix transcription factor), hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor), HIF1-α, HIF-1α, HIF1-ALPHA, HIF-1alpha, HIF-1A, HIF-1 α, HIF1, bHLHe78
Anti-HIF1A Antibody (2X244) 是一种抗体，靶向 HIF1A。Anti-HIF1A Antibody (2X244) 可用于 ELISA, IF, FC。
Anti-HIF1A Antibody (2X244)
Anti-HIF1A Antibody (2X244)
还可以产品编号 TMAH-00543 别名 PASD8, MOP1, hypoxia inducible factor 1, α subunit (basic helix-loop-helix transcription factor), hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor), HIF1-α, HIF-1α, HIF1-ALPHA, HIF-1alpha, HIF-1A, HIF-1 α, HIF1, bHLHe78
Anti-HIF1A Antibody (2X244) 是一种抗体，靶向 HIF1A。Anti-HIF1A Antibody (2X244) 可用于 ELISA, IF, FC。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,310 | 5日内发货 | |
| 100 μL | ¥ 2,185 | 5日内发货 |
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| 产品描述 | Anti-HIF1A Antibody (2X244) is an antibody targeting HIF1A. Anti-HIF1A Antibody (2X244) can be used in ELISA, IF, FC. |
| 别名 | PASD8, MOP1, hypoxia inducible factor 1, α subunit (basic helix-loop-helix transcription factor), hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor), HIF1-α, HIF-1α, HIF1-ALPHA, HIF-1alpha, HIF-1A, HIF-1 α, HIF1, bHLHe78 |
| Ig Type | Rabbit IgG |
| 克隆号 | 2X244 |
| 交叉反应 | Human |
| 验证活性 | 1. Immunofluorescence staining of Hela cell with TMAH-00543 at 1:20, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). 2. Overlay Peak curve showing Hela cells stained with TMAH-00543 (red line) at 1:50. The cells were fixed in 4% formaldehyde (15min) and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Rabbit IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was rabbit IgG1 (1µg/1*10^6 cells) used under the same conditions. Acquisition of >10,001 events was performed. |
| 应用 | ELISA, IF, FC |
| 推荐剂量 | IF:1:20-1:200; FC:1:20-1:200. |
| 抗体种类 | Monoclonal |
| 亚细胞定位 | Cytoplasm. Nucleus. Nucleus speckle. |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | Affinity-chromatography |
| 性状 | Liquid |
| 缓冲液 | Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol, 0.01M PBS, PH 7.4. |
| 研究背景 | Functions as a master transcriptional regulator of the adaptive response to hypoxia. Under hypoxic conditions, activates the transcription of over 40 genes, including erythropoietin, glucose transporters, glycolytic enzymes, vascular endothelial growth factor, HILPDA, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. Plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Heterodimerizes with ARNT; heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hyp... |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein: Human HIF1A Protein |
| 抗原种属 | Human |
| 基因ID | 3091 |
| Uniprot ID | |
| 研究领域 | Epigenetics and Nuclear Signaling, Cancer, Cardiovascular, Metabolism |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
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