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Anti-GDNF Polyclonal Antibody 2
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产品编号 TMAB-00761
Anti-GDNF Polyclonal Antibody 2 是一种 Rabbit 抗体，靶向 GDNF。Anti-GDNF Polyclonal Antibody 2 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-GDNF Polyclonal Antibody 2
Anti-GDNF Polyclonal Antibody 2
还可以产品编号 TMAB-00761
Anti-GDNF Polyclonal Antibody 2 是一种 Rabbit 抗体，靶向 GDNF。Anti-GDNF Polyclonal Antibody 2 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,175 | 5日内发货 | |
| 100 μL | ¥ 1,960 | 5日内发货 | |
| 200 μL | ¥ 2,790 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-GDNF Polyclonal Antibody 2 is a Rabbit antibody targeting GDNF. Anti-GDNF Polyclonal Antibody 2 can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat (predicted:Dog,Pig,Cow,Horse,Sheep) |
| 验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (GDNF) Polyclonal Antibody, Unconjugated (TMAB-00761) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Sample: Cerebrum (Rat) Lysate at 40 μg Cerebellum (Rat) Lysate at 40 μg Primary: Anti-GDNF (TMAB-00761) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 15 kDa Observed band size: 20 kDa 3. Sample: Cerebrum (Mouse) Lysate at 40 μg Primary: Anti-GDNF (TMAB-00761) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 15 kDa Observed band size: 20 kDa 4. Sample: HepG2 (Human) Cell Lysate at 30 μg Primary: Anti-GDNF (TMAB-00761) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 15 kDa Observed band size: 19 kDa |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Secreted. |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | Neurobiology. Neurotrophins. Neuroscience. This gene encodes a highly conserved neurotrophic factor. The recombinant form of this protein was shown to promote the survival and differentiation of dopaminergic neurons in culture, and was able to prevent apoptosis of motor neurons induced by axotomy. The encoded protein is processed to a mature secreted form that exists as a homodimer. The mature form of the protein is a ligand for the product of the RET (rearranged during transfection) protooncogene. In addition to the transcript encoding GDNF, two additional alternative transcripts encoding distinct proteins, referred to as astrocyte-derived trophic factors, have also been described. Mutations in this gene may be associated with Hirschsprung disease. |
| 免疫原 | KLH conjugated synthetic peptide: human GDNF |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Kidney development,Neurotrophins,Amyotrophic lateral sclerosis,Parkinson's disease |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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