Anti-Fibronectin Polyclonal Antibody is a Rabbit antibody targeting Fibronectin. Anti-Fibronectin Polyclonal Antibody can be used in IF,IHC-Fr,IHC-P.

IgG

Human,Mouse,Rat,Cow (predicted:Dog)

1. Tissue/cell: rat intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Fibronectin/FN1 Polyclonal Antibody, Unconjugated (TMAB-00678) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Fibronectin/FN1 Polyclonal Antibody, Unconjugated (TMAB-00678) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Paraformaldehyde-fixed, paraffin embedded (Rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation: Anti-Fibronectin Polyclonal Antibody, conjugated (TMAB-00678-AF647) 1:400, 1.5 hours at 37°C; DAPI (5 μg/ml, blue) was used to stain the cell nucleus.
4. Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Fibronectin) Polyclonal Antibody, Unconjugated (TMAB-00678) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:200-800

Polyclonal

KLH conjugated synthetic peptide: human Fibronectin/Ugl-Y3

Human

FN1

Fibronectin

Extracellular matrix,Extracellular Matrix,Ectoderm,Fibronectin,Ectoderm,Osteogenesis,Extracellular

Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape.
Anastellin binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.