Anti-Fibronectin Polyclonal Antibody is a Rabbit antibody targeting Fibronectin. Anti-Fibronectin Polyclonal Antibody can be used in IHC-P,IHC-Fr,IF.

IgG

Human,Mouse,Rat,Cow (predicted:Dog)

1. Tissue/cell: rat intestine tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Fibronectin/FN1 Polyclonal Antibody, Unconjugated (TMAB-00678) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
2. Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min; Blocking buffer (normal goat serum) at 37°C for 20 min;
Incubation: Anti-Fibronectin/FN1 Polyclonal Antibody, Unconjugated (TMAB-00678) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAb staining.
3. Paraformaldehyde-fixed, paraffin embedded (Rat colon); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Incubation: Anti-Fibronectin Polyclonal Antibody, conjugated (TMAB-00678-AF647) 1:400, 1.5 hours at 37°C; DAPI (5 μg/ml, blue) was used to stain the cell nucleus.
4. Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (Fibronectin) Polyclonal Antibody, Unconjugated (TMAB-00678) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:200-800

Polyclonal

KLH conjugated synthetic peptide: human Fibronectin/Ugl-Y3

Human

Extracellular matrix,Extracellular Matrix,Ectoderm,Fibronectin,Ectoderm,Osteogenesis,Extracellular