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Anti-DLK1 Polyclonal Antibody

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Anti-DLK1 Polyclonal Antibody
产品编号 TMAB-00553

Anti-DLK1 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 DLK1。Anti-DLK1 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。

Anti-DLK1 Polyclonal Antibody

Anti-DLK1 Polyclonal Antibody

Rating icon 还可以
Anti-DLK1 Polyclonal Antibody
产品编号 TMAB-00553

Anti-DLK1 Polyclonal Antibody 是一种 Rabbit 抗体,靶向 DLK1。Anti-DLK1 Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。

规格价格库存数量
50 μL
¥ 1,175
5日内发货
100 μL
¥ 1,975
5日内发货
200 μL
¥ 2,795
5日内发货
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产品介绍

生物活性
产品描述
Anti-DLK1 Polyclonal Antibody is a Rabbit antibody targeting DLK1. Anti-DLK1 Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF.
Ig Type
IgG
交叉反应
Human,Mouse,Rat (predicted:Rabbit,Sheep)
验证活性
1. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (DLK1) Polyclonal Antibody, Unconjugated (TMAB-00553) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.
2. Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Rat Cerebrum tissue lysates
Primary: Anti-DLK1 (TMAB-00553) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 42 kDa
Observed band size: 45 kDa
应用
推荐剂量
WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500
抗体种类
Polyclonal
宿主来源Rabbit
亚细胞定位Membrane; Single-pass type I membrane protein.
构建方式Hybridoma Polyclonal Antibody
纯化方式Protein A purified
性状Liquid
缓冲液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究背景This gene encodes a transmembrane protein containing six epidermal growth factor repeats. The protein is involved in the differentiation of several cell types, including adipocytes; it is also thought to be a tumor suppressor. It is one of several imprinted genes located in a region of on chr 14q32. Certain mutations in this imprinted region can cause phenotypes similar to maternal and paternal uniparental disomy of chromosome 14 (UPD14). This gene is expressed from the paternal allele. A polymorphism within this gene has been associated with child and adolescent obesity. The mode of inheritance for this polymorphism is polar overdominance; this non-Mendelian inheritance pattern was first described in sheep with the callipyge phenotype, which is characterized by muscle hypertrophy and decreased fat mass. [provided by RefSeq, Mar 2010].
抗原信息
免疫原
KLH conjugated synthetic peptide: sheep DLK1
抗原种属
Sheep
研究领域
Hypothalamic pituitary adrenal axis,Neurogenesis,Calcium Binding Proteins,Surface Molecules
存储&运输
储存方式Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles.
运输方式Shipping with blue ice.

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