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Anti-DLAT Polyclonal Antibody
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产品编号 TMAB-00550
Anti-DLAT Polyclonal Antibody 是一种 Rabbit 抗体，靶向 DLAT。Anti-DLAT Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
Anti-DLAT Polyclonal Antibody
Anti-DLAT Polyclonal Antibody
还可以产品编号 TMAB-00550
Anti-DLAT Polyclonal Antibody 是一种 Rabbit 抗体，靶向 DLAT。Anti-DLAT Polyclonal Antibody 可用于 WB,IHC-P,IHC-Fr,IF。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,160 | 5日内发货 | |
| 100 μL | ¥ 1,960 | 5日内发货 | |
| 200 μL | ¥ 2,780 | 5日内发货 |
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产品介绍
生物活性
抗原信息
| 产品描述 | Anti-DLAT Polyclonal Antibody is a Rabbit antibody targeting DLAT. Anti-DLAT Polyclonal Antibody can be used in WB,IHC-P,IHC-Fr,IF. |
| Ig Type | IgG |
| 交叉反应 | Human,Mouse,Rat,Zebrafish (predicted:Dog,Pig,Cow,Horse,Rabbit,Sheep,GuineaPig,Cat) |
| 验证活性 | 1. Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 min; Blocking buffer (normal goat serum) at 37°C for 30 min; Antibody incubation with (PDCE2) Polyclonal Antibody, Unconjugated (TMAB-00550) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining. 2. Sample: Lane 1: Mouse Cerebrum tissue lysates Lane 2: Mouse Heart tissue lysates Lane 3: Mouse Muscle tissue lysates Lane 4: Rat Cerebrum tissue lysates Lane 5: Rat Muscle tissue lysates Lane 6: Human PANC-1 cell lysates Primary: Anti-PDCE2 (TMAB-00550) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 63 kDa Observed band size: 63 kDa |
| 应用 | |
| 推荐剂量 | WB: 1:500-2000; IHC-P: 1:100-500; IHC-Fr: 1:100-500; IF: 1:100-500 |
| 抗体种类 | Polyclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Mitochondrion matrix. |
| 构建方式 | Hybridoma Polyclonal Antibody |
| 纯化方式 | Protein A purified |
| 性状 | Liquid |
| 缓冲液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究背景 | This gene encodes component E2 of the multi-enzyme pyruvate dehydrogenase complex (PDC). PDC resides in the inner mitochondrial membrane and catalyzes the conversion of pyruvate to acetyl coenzyme A. The protein product of this gene, dihydrolipoamide acetyltransferase, accepts acetyl groups formed by the oxidative decarboxylation of pyruvate and transfers them to coenzyme A. Dihydrolipoamide acetyltransferase is the antigen for antimitochondrial antibodies. These autoantibodies are present in nearly 95% of patients with the autoimmune liver disease primary biliary cirrhosis (PBC). In PBC, activated T lymphocytes attack and destroy epithelial cells in the bile duct where this protein is abnormally distributed and overexpressed. PBC enventually leads to cirrhosis and liver failure. Mutations in this gene are also a cause of pyruvate dehydrogenase E2 deficiency which causes primary lactic acidosis in infancy and early childhood.[provided by RefSeq, Oct 2009] |
| 免疫原 | KLH conjugated synthetic peptide: human PDCE2 |
| 抗原种属 | Human |
| 基因ID | |
| Uniprot ID | |
| 研究领域 | Metabolism of carbohydrates,Carbohydrate metabolism,Energy Metabolism,Mitochondrial markers,Cancer,Energy Metabolism,Mitochondrial |
存储&运输
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
计算器
比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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