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别名 mitochondrial, CPT2, CPT1, Carnitine palmitoyltransferase II (CPT II), Carnitine O-palmitoyltransferase 2, mitochondrial
Anti-CPT2 Antibody (3I417) 是一种 Rabbit 抗体,靶向 CPT2。Anti-CPT2 Antibody (3I417) 可用于 ICC/IF,IHC,WB。
Anti-CPT2 Antibody (3I417) 是一种 Rabbit 抗体,靶向 CPT2。Anti-CPT2 Antibody (3I417) 可用于 ICC/IF,IHC,WB。
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 50 μL | ¥ 1,480 | 5日内发货 | |
| 100 μL | ¥ 2,490 | 5日内发货 |
| 产品描述 | Anti-CPT2 Antibody (3I417) is a Rabbit antibody targeting CPT2. Anti-CPT2 Antibody (3I417) can be used in ICC/IF,IHC,WB. |
| 别名 | mitochondrial, CPT2, CPT1, Carnitine palmitoyltransferase II (CPT II), Carnitine O-palmitoyltransferase 2, mitochondrial |
| Ig Type | IgG |
| 克隆号 | 3I417 |
| 反应种属 | Human,Mouse,Rat |
| 验证活性 | 1. Western blot analysis of CPT2 on different lysates using anti-CPT2 antibody at 1/1,000 dilution. Positive control: Lane 1: Hela, Lane 2: 293, Lane 3: HepG2, Lane 4: NIH/3T3. 2. Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-CPT2 antibody. Counter stained with hematoxylin. 3. Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CPT2 antibody. Counter stained with hematoxylin. 4. Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-CPT2 antibody. Counter stained with hematoxylin. 5. ICC staining CPT2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. 6. ICC staining CPT2 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. ![]() ![]() ![]() ![]() ![]() ![]() |
| 应用 | ICC/IFIHCWB |
| 推荐剂量 | WB: 1:500-1000; IHC: 1:50-200; ICC/IF: 1:50-200 |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 构建方式 | Recombinant Antibody |
| 纯化方式 | ProA affinity purified |
| 性状 | Liquid |
| 缓冲液 | 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. |
| 研究背景 | The mitochondrial β-oxidation of long-chain fatty acids is initiated by the sequential action of carnitine palmitoyltransferase (CPT) I (outer membrane and detergent labile) and II (inner membrane and detergent stable), together with carnitine carrier. CPTI catalyzes the first reaction in the transport of long-chain fatty acids from the cytoplasm to the mitochondrion, a rate-limiting step in beta-oxidation. Two types of CPTI are known, the liver (CPTIA) and muscle (CPTIB) isoforms. The muscle type protein is specially expressed in heart and skeletal muscle. Membrane-bound CPTI, but not CPTII, is inhibited reversibly by malonyl-coenzyme A (CoA). Unlike CPTII, CPTI requires membrane integrity for catalytic function. In addition, glutamic acid 3 and histidine 5 are necessary for malonyl CoA inhibition and binding to liver CPTI, but not for catalytic activity. |
| 偶联 | Unconjugated |
| 免疫原 | Recombinant Protein |
| Uniprot ID |
| 分子量 | Theoretical: 74 kDa. |
| 储存方式 | Store at -20°C or -80°C for 12 months. Avoid repeated freeze-thaw cycles. |
| 运输方式 | Shipping with blue ice. |
| 存储 | store at low temperature | -20°C for 1 year |